BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 20046

Title: SOLUTION NMR STRUCTURE OF RAT IAPP-1-19   PubMed: 18989932

Authors: NANGA, RAVI PRAKASH REDDY; BRENDER, JEFFREY; XU, JIADI; VEGLIA, GIANLUIGI; RAMAMOORTHY, AYYALUSAMY

Citation: NANGA, RAVI PRAKASH REDDY; BRENDER, JEFFREY; XU, JIADI; VEGLIA, GIANLUIGI; RAMAMOORTHY, AYYALUSAMY. "Structures of rat and human islet amyloid polypeptide IAPP(1-19) in micelles by NMR spectroscopy"  Biochemistry 47, 12689-12697 (2008).

Assembly members:
IAPP, polymer, 19 residues, Formula weight is not available

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
IAPP: KCNTATCATQRLANFLVRS

Data sets:
Data typeCount
1H chemical shifts106

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1IAPP1

Entities:

Entity 1, IAPP 19 residues - Formula weight is not available

1   LYSCYSASNTHRALATHRCYSALATHRGLN
2   ARGLEUALAASNPHELEUVALARGSER

Samples:

sample_1: Peptide 1.625 mM; DPC, [U-100% 2H], 200 mM; sodium azide 0.02%; sodium phosphate 20 mM; sodium chloride 120 mM; D2O 10%; H2O 90%

sample_conditions_1: pH: 7.3; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

TOPSPIN v2.1, Bruker Biospin - collection, processing

SPARKY, Goddard - chemical shift assignment, chemical shift calculation, data analysis

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure solution

TALOS, Cornilescu, Delaglio and Bax - refinement

ProcheckNMR, Laskowski and MacArthur - data analysis

NMR spectrometers:

  • Bruker Avance 900 MHz

Related Database Links:

BMRB 16314
PDB 2KJ7
DBJ BAA22051
EMBL CAA37003
GB AAA37874 AAA40730 AAA41359 AAH27527 ACU29638
PRF 1802238A
REF NP_034621 NP_036718 XP_001098180 XP_001144800 XP_002926618
SP P12968 P12969