BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 20045

Title: SOLUTION NMR STRUCTURE OF HUMAN IAPP-1-19   PubMed: 18989932

Authors: NANGA, RAVI PRAKASH REDDY; BRENDER, JEFFREY; XU, JIADI; VEGLIA, GIANLUIGI; RAMAMOORTHY, AYYALUSAMY

Citation: NANGA, RAVI PRAKASH REDDY; BRENDER, JEFFREY; XU, JIADI; VEGLIA, GIANLUIGI; RAMAMOORTHY, AYYALUSAMY. "Structures of rat and human islet amyloid polypeptide IAPP(1-19) in micelles by NMR spectroscopy"  Biochemistry 47, 12689-12697 (2008).

Assembly members:
IAPP, polymer, 19 residues, 2081.410 Da.

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
IAPP: KCNTATCATQRLANFLVHS

Data sets:
Data typeCount
1H chemical shifts104

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1IAPP1

Entities:

Entity 1, IAPP 19 residues - 2081.410 Da.

1   LYSCYSASNTHRALATHRCYSALATHRGLN
2   ARGLEUALAASNPHELEUVALHISSER

Samples:

sample_1: entity 1.625 mM; DPC, [U-100% 2H], 200 mM; sodium azide 0.02%; sodium phosphate 20 mM; sodium chloride 120 mM; D2O 10%; H2O 90%

sample_conditions_1: pH: 7.3; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1

Software:

TOPSPIN v2.1, Bruker Biospin - collection, processing

SPARKY, Goddard - chemical shift assignment, chemical shift calculation, data analysis

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure solution

TALOS, Cornilescu, Delaglio and Bax - refinement

ProcheckNMR, Laskowski and MacArthur - data analysis

NMR spectrometers:

  • Bruker Avance 900 MHz

Related Database Links:

BMRB 16104 16105 17394 18795
PDB
DBJ BAG73319
EMBL CAA33032 CAA37002 CAA39504 CAA39545 CAA48724
GB AAA35524 AAA35983 AAA51728 AAA52281 AAI11850
REF NP_000406 NP_001268503
SP P10997 P19890 P23442