BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 19999

Title: 1H, 15N, 13C resonance assignment of human osteopontin   PubMed: 25616494

Deposition date: 2014-06-02 Original release date: 2019-07-11

Authors: Platzer, Gerald; Zerko, Szymon; Kozminski, Wiktor; Konrat, Robert

Citation: Platzer, Gerald; Zerko, Szymon; Saxena, Saurabh; Kozminski, Wiktor; Konrat, Robert. "1H, 15N, 13C resonance assignment of human osteopontin"  Biomol. NMR Assign. 9, 289-292 (2015).

Assembly members:
osteopontin, polymer, 302 residues, Formula weight is not available

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
osteopontin: GAMGIPVKQADSGSSEEKQL YNKYPDAVATWLNPDPSQKQ NLLAPQNAVSSEETNDFKQE TLPSKSNESHDHMDDMDDED DDDHVDSQDSIDSNDSDDVD DTDDSHQSDESHHSDESDEL VTDFPTDLPATEVFTPVVPT VDTYDGRGDSVVYGLRSKSK KFRRPDIQYPDATDEDITSH MESEELNGAYKAIPVAQDLN APSDWDSRGKDSYETSQLDD QSAETHSHKQSRLYKRKAND ESNEHSDVIDSQELSKVSRE FHSHEFHSHEDMLVVDPKSK EEDKHLKFRISHELDSASSE VN

Data sets:
Data typeCount
13C chemical shifts902
15N chemical shifts257
1H chemical shifts1147

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1osteopontin1

Entities:

Entity 1, osteopontin 302 residues - Formula weight is not available

1   GLYALAMETGLYILEPROVALLYSGLNALA
2   ASPSERGLYSERSERGLUGLULYSGLNLEU
3   TYRASNLYSTYRPROASPALAVALALATHR
4   TRPLEUASNPROASPPROSERGLNLYSGLN
5   ASNLEULEUALAPROGLNASNALAVALSER
6   SERGLUGLUTHRASNASPPHELYSGLNGLU
7   THRLEUPROSERLYSSERASNGLUSERHIS
8   ASPHISMETASPASPMETASPASPGLUASP
9   ASPASPASPHISVALASPSERGLNASPSER
10   ILEASPSERASNASPSERASPASPVALASP
11   ASPTHRASPASPSERHISGLNSERASPGLU
12   SERHISHISSERASPGLUSERASPGLULEU
13   VALTHRASPPHEPROTHRASPLEUPROALA
14   THRGLUVALPHETHRPROVALVALPROTHR
15   VALASPTHRTYRASPGLYARGGLYASPSER
16   VALVALTYRGLYLEUARGSERLYSSERLYS
17   LYSPHEARGARGPROASPILEGLNTYRPRO
18   ASPALATHRASPGLUASPILETHRSERHIS
19   METGLUSERGLUGLULEUASNGLYALATYR
20   LYSALAILEPROVALALAGLNASPLEUASN
21   ALAPROSERASPTRPASPSERARGGLYLYS
22   ASPSERTYRGLUTHRSERGLNLEUASPASP
23   GLNSERALAGLUTHRHISSERHISLYSGLN
24   SERARGLEUTYRLYSARGLYSALAASNASP
25   GLUSERASNGLUHISSERASPVALILEASP
26   SERGLNGLULEUSERLYSVALSERARGGLU
27   PHEHISSERHISGLUPHEHISSERHISGLU
28   ASPMETLEUVALVALASPPROLYSSERLYS
29   GLUGLUASPLYSHISLEULYSPHEARGILE
30   SERHISGLULEUASPSERALASERSERGLU
31   VALASN

Samples:

nmr_sample: sodium phosphate 50 mM; sodium chloride 50 mM; osteopontin, [U-95% 13C; U-95% 15N], 0.5 mM; H2O 90%; D2O, [U-100% 2H], 10%

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
3D HNCOnmr_sampleisotropicsample_conditions_1
4D HN(CA)NHnmr_sampleisotropicsample_conditions_1
5D HN(CA)CONHnmr_sampleisotropicsample_conditions_1
5D (HACA)CON(CA)CONHnmr_sampleisotropicsample_conditions_1
5D (H)NCO(NCA)CONHnmr_sampleisotropicsample_conditions_1
5D HNCOCACBnmr_sampleisotropicsample_conditions_1

Software:

VNMRJ, Varian - collection

NMR spectrometers:

  • Agilent Direct Drive 700 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts