BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 19993

Title: Lysyl t-RNA synthetase 1-72   PubMed: 24983501

Authors: Mushtaq, Ameeq; Cho, Hye; Jeon, Young

Citation: Cho, Hye Young; Ul Mushtaq, Ameeq; Lee, Jin Young; Kim, Dae Gyu; Seok, Min Sook; Jang, Minseok; Han, Byung-Woo; Kim, Sunghoon; Jeon, Young Ho. "Characterization of the interaction between lysyl-tRNA synthetase and laminin receptor by NMR"  FEBS Lett. 588, 2851-2858 (2014).

Assembly members:
LysRS1-72, polymer, 72 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
LysRS1-72: MAAVQAAEVKVDGSEPKLSK NELKRRLKAEKKVAEKEAKQ KELSEKQLSQATAAATNHTT DNGVGPEEESVD

Data sets:
Data typeCount
13C chemical shifts166
15N chemical shifts61
1H chemical shifts226
T1 relaxation values43
T2 relaxation values43
heteronuclear NOE values40

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Lysyl t-RNA synthetase1-721

Entities:

Entity 1, Lysyl t-RNA synthetase1-72 72 residues - Formula weight is not available

1   METALAALAVALGLNALAALAGLUVALLYS
2   VALASPGLYSERGLUPROLYSLEUSERLYS
3   ASNGLULEULYSARGARGLEULYSALAGLU
4   LYSLYSVALALAGLULYSGLUALALYSGLN
5   LYSGLULEUSERGLULYSGLNLEUSERGLN
6   ALATHRALAALAALATHRASNHISTHRTHR
7   ASPASNGLYVALGLYPROGLUGLUGLUSER
8   VALASP

Samples:

sample_1: LysRS residues 1-72, [U-13C; U-15N], 0.2 mM; sodium chloride 100 mM; HEPES 20 mM; DTT 1 mM; PMSF 1 mM; D2O 10%; H2O 90%

sample_2: LysRS residues 1-72, [U-15N], 0.2 mM; sodium chloride 100 mM; HEPES 20 mM; DTT 1 mM; PMSF 1 mM; D2O 10%; H2O 90%

sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HCACOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HBHANHsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
2D 1H-15N HSQC -T2sample_2isotropicsample_conditions_1
2D 1H-15N HSQC-T1sample_2isotropicsample_conditions_1
2D 1H-15N HSQC-NOEsample_2isotropicsample_conditions_1

Software:

CCPN v2.1.5, CCPN, Goddard - Relaxation data analysis, chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz

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