BMRB Entry 19952

Title:
Solution structure of StAR-related lipid transfer domain protein 6 (STARD6)
Deposition date:
2014-05-05
Original release date:
2014-05-20
Authors:
Letourneau, Danny; Bedard, Mikael; Lefebvre, Andree; LeHoux, Jean-Guy; Lavigne, Pierre
Citation:

Citation: Letourneau, Danny; Bedard, Mikael; Lefebvre, Andree; LeHoux, Jean-Guy; Lavigne, Pierre. "Solution structure of StAR-related lipid transfer domain protein 6 (STARD6)"  .

Assembly members:

Assembly members:
StAR_D6, polymer, 220 residues, 25037.572 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21b

Data sets:
Data typeCount
13C chemical shifts878
15N chemical shifts201
1H chemical shifts1355

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1StAR D61

Entities:

Entity 1, StAR D6 220 residues - 25037.572 Da.

1   METASPPHELYSALAILEALAGLNGLNTHR
2   ALAGLNGLUVALLEUGLYTYRASNARGASP
3   THRSERGLYTRPLYSVALVALLYSTHRSER
4   LYSLYSILETHRVALSERSERLYSALASER
5   ARGLYSPHEHISGLYASNLEUTYRARGVAL
6   GLUGLYILEILEPROGLUSERPROALALYS
7   LEUSERASPPHELEUTYRGLNTHRGLYASP
8   ARGILETHRTRPASPLYSSERLEUGLNVAL
9   TYRASNMETVALHISARGILEASPSERASP
10   THRPHEILECYSHISTHRILETHRGLNSER
11   PHEALAVALGLYSERILESERPROARGASP
12   PHEILEASPLEUVALTYRILELYSARGTYR
13   GLUGLYASNMETASNILEILESERSERLYS
14   SERVALASPPHEPROGLUTYRPROPROSER
15   SERASNTYRILEARGGLYTYRASNHISPRO
16   CYSGLYPHEVALCYSSERPROMETGLUGLU
17   ASNPROALATYRSERLYSLEUVALMETPHE
18   VALGLNTHRGLUMETARGGLYLYSLEUSER
19   PROSERILEILEGLULYSTHRMETPROSER
20   ASNLEUVALASNPHEILELEUASNALALYS
21   ASPGLYILELYSALAHISARGTHRPROSER
22   ARGARGGLYPHEHISHISASNSERHISSER

Samples:

STARD6: StAR_D6, [U-100% 13C; U-100% 15N], 1 mM

CondSet1: ionic strength: 0.100 M; pH: 7.000; pressure: 1.000 atm; temperature: 298.150 K

Experiments:

NameSampleSample stateSample conditions
3D HNCACBSTARD6isotropicCondSet1
3D C(CO)NHSTARD6isotropicCondSet1
3D HNCOSTARD6isotropicCondSet1
2D 1H-15N HSQCSTARD6isotropicCondSet1
3D HN(CO)CASTARD6isotropicCondSet1
3D HNHASTARD6isotropicCondSet1
3D H(CCO)NHSTARD6isotropicCondSet1
3D 1H-15N NOESYSTARD6isotropicCondSet1
3D HCCH-TOCSYSTARD6isotropicCondSet1
2D 1H-13C HSQCSTARD6isotropicCondSet1
3D 1H-13C NOESY aliphaticSTARD6isotropicCondSet1
2D 1H-13C HSQC aromaticSTARD6isotropicCondSet1
3D 1H-13C NOESY aromaticSTARD6isotropicCondSet1
2D 1H-13C HSQC aliphaticSTARD6isotropicCondSet1

Software:

CNS v1.21, Brunger, Adams, Clore, Gros, Nilges and Read - Structure calculation, refinement

CcpNmr_Analysis v2.2.2, CCPN - Assignment

DANGLE v1.1, CCPN - Dihedral Restraints from Chemical Shifts

NMR_Pipe v2.0, NMR Pipe - file conversion

ARIA v2.2, Nilges, Rieping, Habeck, Bardiaux, Bernard and Malliavin - Structure calculation, refinement

NMR spectrometers:

  • Varian UnityInova 600 MHz

Related Database Links:

UniProt P59095
BMRB 18777
PDB
DBJ BAI46405
GB AAI40309 AAI46469 AAL89655 EAW63002 EAW63004
REF NP_631910 XP_011524122 XP_011524123 XP_014197853 XP_523930
SP P59095
AlphaFold P59095 P59095

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks