BMRB Entry 19891

Name: A Mechanism for the cAMP-Dependent Auto-Inhibition of HCN
Published: 2016-06-17

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PDB ID: 2mng
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19891
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

A Mechanism for the cAMP-Dependent Auto-Inhibition of HCN

Deposition date:

2014-04-03

Original release date:

2016-06-17

Authors:

Akimoto, Madoka; Zhang, Zaiyong; Boulton, Stephen; Selvaratnam, Rajeevan; VanSchouwen, Bryan; Gloyd, Melanie; Accili, Eric; Lange, Oliver; Melacini, Giuseppe

Citation:

Citation: Akimoto, Madoka; Zhang, Zaiyong; Boulton, Stephen; Selvaratnam, Rajeevan; VanSchouwen, Bryan; Gloyd, Melanie; Accili, Eric; Lange, Oliver; Melacini, Giuseppe. "A mechanism for the auto-inhibition of hyperpolarization-activated cyclic nucleotide-gated (HCN) channel opening and its relief by cAMP" J. Biol. Chem. 289, 22205-22220 (2014).
PubMed: 24878962

Assembly members:

Assembly members:
Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4, polymer, 131 residues, 14985.341 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET302NT-His

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4: NSEEIINFNCRKLVASMPLF ANADPNFVTSMLTKLRFEVF QPGDYIIREGTIGKKMYFIQ HGVVSVLTKGNKETKLADGS YFGEICLLTRGRRTASVRAD TYCRLYSLSVDNFNEVLEEY PMMRRAFETVA

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	459
15N chemical shifts	118
1H chemical shifts	666

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4	1

Entities:

Entity 1, Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4 131 residues - 14985.341 Da.

Residues 577N, 578S(First two residues) represent non-native amino acids from cleavage site.

1

ASN

SER

GLU

ILE

ASN

PHE

ASN

CYS

2

ARG

LYS

LEU

VAL

ALA

SER

MET

PRO

LEU

PHE

3

ALA

ASN

ALA

ASP

PRO

ASN

PHE

VAL

THR

SER

4

MET

LEU

THR

LYS

LEU

ARG

PHE

GLU

VAL

PHE

5

GLN

PRO

GLY

ASP

TYR

ILE

ARG

GLU

GLY

6

THR

ILE

GLY

LYS

MET

TYR

PHE

ILE

GLN

7

HIS

GLY

VAL

SER

VAL

LEU

THR

LYS

GLY

8

ASN

LYS

GLU

THR

LYS

LEU

ALA

ASP

GLY

SER

9

TYR

PHE

GLY

GLU

ILE

CYS

LEU

THR

ARG

10

GLY

ARG

THR

ALA

SER

VAL

ARG

ALA

ASP

11

THR

TYR

CYS

ARG

LEU

TYR

SER

LEU

SER

VAL

12

ASP

ASN

PHE

ASN

GLU

VAL

LEU

GLU

TYR

13

PRO

MET

ARG

ALA

PHE

GLU

THR

VAL

14

ALA

Samples:

sample_1: DTT 1 mM; MES 20 mM; potassium chloride 100 mM; EDTA 2 mM; EGTA 2 mM; sodium azide 0.02%; Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4, [U-13C; U-15N], 0.5 mM; D2O 95%; H2O 5%

sample_2: DTT 1 mM; MES 20 mM; potassium chloride 100 mM; EDTA 1 mM; sodium azide 0.02%; Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4, [U-15N], 0.2 mM; Pf1 phage 6 mg/mL; D2O 95%; H2O 5%

sample_3: DTT 1 mM; MES 20 mM; potassium chloride 100 mM; EDTA 1 mM; sodium azide 0.02%; Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4, [U-15N], 0.2 mM; D2O 95%; H2O 5%

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 300 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_2	anisotropic	sample_conditions_1
2D 1H-15N HSQC	sample_3	anisotropic	sample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

Bruker Avance 700 MHz
Bruker Avance 850 MHz

NCBI	NP_005468
UNP	Q9Y3Q4
AlphaFold	Q9UMQ7