BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 19873

Title: D loop of tRNA(Met)   PubMed: 25815904

Authors: Eveline, Lescrinier; Natala, Dyubankova; Piet, Herdewijn

Citation: Eveline, Lescrinier; Natalia, Dyubankova; Piet, Herdewijn. "Contribution of dihydrouridine in folding of the D-arm in tRNA"  Org. Biomol. Chem. ., .-. (2015).

Assembly members:
D_arm_tRNA(Met), polymer, 16 residues, Formula weight is not available

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
D_arm_tRNA(Met): GGAGAGXGGAACUCCC

Data sets:
Data typeCount
13C chemical shifts94
1H chemical shifts110

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1D-arm of tRNA(Met)1

Entities:

Entity 1, D-arm of tRNA(Met) 16 residues - Formula weight is not available

1   GGAGAGH2UGGA
2   ACUCCC

Samples:

sample_1: D arm tRNA(Met) 1 ± 0.5 mM; D2O 100%

sample_conditions_1: pH: 6; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

TOPSPIN v2.1, Bruker Biospin - data analysis

X-PLOR_NIH v3.851, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMR spectrometers:

  • Bruker Avance 600 MHz

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