BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 19848

Title: NMR solution structure of PA3793 from Pseudomonas aeruginosa

Deposition date: 2014-03-11 Original release date: 2014-04-28

Authors: Mayzel, M.; Schnell, R.; Schneider, G.; Karlsson, Goran

Citation: Schneider, G.; Schnell, R.; Mayzel, M.; Karlsson, Goran. "NMR SOLUTION STRUCTURE OF PA3793 FROM PSEUDOMONAS"  Not known ., .-..

Assembly members:
UNCHARACTERIZED_PROTEIN, polymer, 113 residues, 12725.7071 Da.

Natural source:   Common Name: Pseudomonas aeruginosa   Taxonomy ID: 287   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Pseudomonas aeruginosa

Experimental source:   Production method: recombinant technology   Host organism: E. coli

Entity Sequences (FASTA):
UNCHARACTERIZED_PROTEIN: GSHMTTQRYLLDELETADML EIDGLHAWRFELNENLLDQA DLAAEADQPFASEDWVLAVE SLDGRTRREWRFSYNAVMEA EPQADGESWRLTTGEGAYQL RCLGAVSASGEDE

Data sets:
Data typeCount
13C chemical shifts462
15N chemical shifts120
1H chemical shifts729

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PA3793 from Pseudomonas aeruginosa1

Entities:

Entity 1, PA3793 from Pseudomonas aeruginosa 113 residues - 12725.7071 Da.

1   GLYSERHISMETTHRTHRGLNARGTYRLEU
2   LEUASPGLULEUGLUTHRALAASPMETLEU
3   GLUILEASPGLYLEUHISALATRPARGPHE
4   GLULEUASNGLUASNLEULEUASPGLNALA
5   ASPLEUALAALAGLUALAASPGLNPROPHE
6   ALASERGLUASPTRPVALLEUALAVALGLU
7   SERLEUASPGLYARGTHRARGARGGLUTRP
8   ARGPHESERTYRASNALAVALMETGLUALA
9   GLUPROGLNALAASPGLYGLUSERTRPARG
10   LEUTHRTHRGLYGLUGLYALATYRGLNLEU
11   ARGCYSLEUGLYALAVALSERALASERGLY
12   GLUASPGLU

Samples:

sample_1: UNCHARACTERIZED PROTEIN, [U-13C; U-15N], 1.6 mM

sample_2: UNCHARACTERIZED PROTEIN, [U-13C; U-15N], 1.6 mM

sample_3: UNCHARACTERIZED PROTEIN 1.6 mM

sample_conditions_1: ionic strength: 70.000 mM; pH: 6.000; pressure: 1.000 atm; temperature: 298.000 K

Experiments:

NameSampleSample stateSample conditions
15Nnoesysample_1solutionsample_conditions_1
15Nhsqcsample_1solutionsample_conditions_1
backbone expssample_1solutionsample_conditions_1
13C noesysample_2solutionsample_conditions_1
2D noesysample_3solutionsample_conditions_1
2D tocsysample_3solutionsample_conditions_1

Software:

AutoDep v4.3, PDBe - collection

ccpn vany, CCPN - chemical shift assignment

cns-1.3 vany, Brunger, Adams, Clore, Gros, Nilges and Read - chemical shift assignment

NMR spectrometers:

  • Varian UnityInova 900 MHz

Related Database Links:

UNP Q9HXK4 B7UXH5_PSEA8
PDB
DBJ BAK91803 BAP20306 BAP49165 BAQ37993 BAR66104
EMBL CAW25908 CCQ86427 CDH69504 CDH75587 CDI92248
GB AAG07180 AAT49972 ABJ13055 AEO73632 AFM63365
REF NP_252482 WP_003092770 WP_003122102 WP_003137861 WP_015502428

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts