BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 19831

Title: 13C and 15N Chemical Shift Assignments for the cellulose-binding protein CBM3b-Cbh9A

Authors: Ivanir, Hadar; Goldbourt, Amir

Citation: Ivanir, Hadar; Goldbourt, Amir. "Solid state NMR chemical shift assignment and conformational analysis of a cellulose binding protein facilitated by optimized glycerol enrichment"  J. Biomol. NMR ., .-..

Assembly members:
CBM3b-Cbh9A, polymer, 166 residues, 18662.6 Da.

Natural source:   Common Name: Clostridium thermocellum   Taxonomy ID: 1515   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Clostridium thermocellum

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
CBM3b-Cbh9A: MGSSHHHHHHSSGLVPRGSH MDVKVQYLCENTQTSTQEIK GKFNIVNTGNRDYSLKDIVL RYYFTKEHNSQLQFICYYTP IGSGNLIPSFGGSGDEHYLQ LEFKDVKLPAGGQTGEIQFV IRYADNSFHDQSNDYSFDPT IKAFQDYGKVTLYKNGELVW GTPPGG

Data sets:
Data typeCount
13C chemical shifts504
15N chemical shifts129

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1CBM3b-Cbh9A1

Entities:

Entity 1, CBM3b-Cbh9A 166 residues - 18662.6 Da.

Residues 1-20 represent a disordered histidine purification tag

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLEUVALPROARGGLYSERHIS
3   METASPVALLYSVALGLNTYRLEUCYSGLU
4   ASNTHRGLNTHRSERTHRGLNGLUILELYS
5   GLYLYSPHEASNILEVALASNTHRGLYASN
6   ARGASPTYRSERLEULYSASPILEVALLEU
7   ARGTYRTYRPHETHRLYSGLUHISASNSER
8   GLNLEUGLNPHEILECYSTYRTYRTHRPRO
9   ILEGLYSERGLYASNLEUILEPROSERPHE
10   GLYGLYSERGLYASPGLUHISTYRLEUGLN
11   LEUGLUPHELYSASPVALLYSLEUPROALA
12   GLYGLYGLNTHRGLYGLUILEGLNPHEVAL
13   ILEARGTYRALAASPASNSERPHEHISASP
14   GLNSERASNASPTYRSERPHEASPPROTHR
15   ILELYSALAPHEGLNASPTYRGLYLYSVAL
16   THRLEUTYRLYSASNGLYGLULEUVALTRP
17   GLYTHRPROPROGLYGLY

Samples:

sample_1: CBM3b-Cbh9A, [2-13C] glycerol, 30 mg/mL

sample_2: CBM3b-Cbh9A, [U-100% 13C; U-100% 15N], 30 mg/mL

sample_conditions_1: ionic strength: 0.4 M; pH: 7.5; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 13C-13C DARRsample_1solidsample_conditions_1
2D 15N-15N PDSDsample_1solidsample_conditions_1
2D 13C-13C DARRsample_2solidsample_conditions_1
3D NCACXsample_1solidsample_conditions_1
3D NCOCXsample_1solidsample_conditions_1
2D NCAsample_1solidsample_conditions_1
3D NCACXsample_2solidsample_conditions_1

Software:

SPARKY v3.113, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance-III 600 MHz

Related Database Links:

PDB
EMBL CAA56918 CDG35109
GB ABN51651 ADU74864 EEU00446 EFB38789 EIC05226
REF WP_003512736 WP_003519027