BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 19765

Title: gbvb5   PubMed: 24741107

Authors: Montserret, Roland; Martin, Annette; Penin, Francois

Citation: Boukadida, Celia; Marnata, Caroline; Montserret, Roland; Cohen, Lisette; Blumen, Brigitte; Gouttenoire, Jerome; Moradpour, Darius; Penin, Francois; Martin, Annette. "NS2 Proteins of GB Virus B and Hepatitis C Virus Share Common Protease Activities and Membrane Topologies."  J. Virol. 88, 7426-7444 (2014).

Assembly members:
entity, polymer, 25 residues, 3116.649 Da.

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: GBVB virus

Experimental source:   Production method: chemical synthesis   Host organism: none

Entity Sequences (FASTA):
entity: HAWYSHYVLKFFLLVFGENG VFFYK

Data sets:
Data typeCount
1H chemical shifts175

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1gbvb51

Entities:

Entity 1, gbvb5 25 residues - 3116.649 Da.

1   HISALATRPTYRSERHISTYRVALLEULYS
2   PHEPHELEULEUVALPHEGLYGLUASNGLY
3   VALPHEPHETYRLYS

Samples:

sample_1: entity 2 mM; TFE, [U-99% 2H], 50 % v/v; H2O 50 % v/v

sample_conditions_1: ionic strength: 0 M; pH: 6; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

SPARKY, Goddard - chemical shift assignment, data analysis

X-PLOR_NIH v2.34, Schwieters, Kuszewski, Tjandra and Clore - structure solution

Molmol, Koradi, Billeter and Wuthrich - data analysis, structure solution

NMR spectrometers:

  • Bruker Avance 500 MHz
  • Bruker Avance 1000 MHz

Related Database Links:

UNP Q69422
PDB
DBJ BAK24070 BAK24071 BAK24072 BAK24073 BAK24074
EMBL CAC33083
GB AAC54059 AAF01368 AAP57528 AGW83695 AGW83696
PRF 2111488B 2119353B
REF NP_056931 NP_757356
SP Q69422