BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 19745

Title: Solution NMR structure of a mismatch DNA

Authors: Ghosh, Anirban; Kar, Rajiv; Bhunia, Anirban; Chatterjee, Subhrangsu

Citation: Ghosh, Anirban; Kar, Rajiv; Chatterjee, Subhrangsu; Bhunia, Anirban. "Solution NMR structure of mismatch DNA"  Not known ., .-..

Assembly members:
DNA_(28-MER)_1, polymer, 14 residues, 8608.586 Da.
DNA_(28-MER)_2, polymer, 14 residues, 8608.586 Da.

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
DNA_(28-MER)_1: GCGCATGCTACGCG
DNA_(28-MER)_2: CGCGTACGATGCGC

Data sets:
Data typeCount
1H chemical shifts212

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1DNA (28-MER), 11
2DNA (28-MER), 22

Entities:

Entity 1, DNA (28-MER), 1 14 residues - 8608.586 Da.

1   DGDCDGDCDADTDGDCDTDA
2   DCDGDCDG

Entity 2, DNA (28-MER), 2 14 residues - 8608.586 Da.

1   DCDGDCDGDTDADCDGDADT
2   DGDCDGDC

Samples:

sample_1: DNA (28-MER) 1 1 mM; D2O 90%; H2O 10%; DNA (28-MER) 2 1 mM

sample_conditions_1: pH: 7.2; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

AMBER, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollman - geometry optimization, refinement

NMR spectrometers:

  • Bruker DRX 500 MHz

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