BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 19715

Title: 13C, 15N and 1H backbone and sidechain chemical shift assignment of receiver domain of ethylene receptor ETR1   PubMed: 24696326

Deposition date: 2014-01-02 Original release date: 2014-04-14

Authors: Hung, Yi-Lin; Sue, Shih-Che

Citation: Hung, Yi-Lin; Lin, Yi-Jan; Sue, Shih-Che. "(13)C, (15)N and (1)H resonance assignments of receiver domain of ethylene receptor ETR1."  Biomol. NMR Assignments ., .-. (2014).

Assembly members:
Receiver_domain_of_ethylene_receptor_ETR1, polymer, 144 residues, Formula weight is not available

Natural source:   Common Name: Thale cress   Taxonomy ID: 3702   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Arabidopsis thaliana

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Receiver_domain_of_ethylene_receptor_ETR1: MHHHHHHAMGSNFTGLKVLV MDENGVSRMVTKGLLVHLGC EVTTVSSNEECLRVVSHEHK VVFMDVCMPGVENYQIALRI HEKFTKQRHQRPLLVALSGN TDKSTKEKCMSFGLDGVLLK PVSLDNIRDVLSDLLEPRVL YEGM

Data sets:
Data typeCount
13C chemical shifts539
15N chemical shifts136
1H chemical shifts838

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Receiver domain of ethylene receptor ETR11

Entities:

Entity 1, Receiver domain of ethylene receptor ETR1 144 residues - Formula weight is not available

Residues 1-10 represent a non-native affinity tag.

1   METHISHISHISHISHISHISALAMETGLY
2   SERASNPHETHRGLYLEULYSVALLEUVAL
3   METASPGLUASNGLYVALSERARGMETVAL
4   THRLYSGLYLEULEUVALHISLEUGLYCYS
5   GLUVALTHRTHRVALSERSERASNGLUGLU
6   CYSLEUARGVALVALSERHISGLUHISLYS
7   VALVALPHEMETASPVALCYSMETPROGLY
8   VALGLUASNTYRGLNILEALALEUARGILE
9   HISGLULYSPHETHRLYSGLNARGHISGLN
10   ARGPROLEULEUVALALALEUSERGLYASN
11   THRASPLYSSERTHRLYSGLULYSCYSMET
12   SERPHEGLYLEUASPGLYVALLEULEULYS
13   PROVALSERLEUASPASNILEARGASPVAL
14   LEUSERASPLEULEUGLUPROARGVALLEU
15   TYRGLUGLYMET

Samples:

sample_Receiver_domain_of_ethylene_receptor_ETR1: ETR1-RD, [U-100% 13C; U-100% 15N], 0.5 mM; sodium chloride 150 mM; TRIS 40 mM

sample_condition_1: pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_Receiver_domain_of_ethylene_receptor_ETR1isotropicsample_condition_1
2D 1H-13C HSQCsample_Receiver_domain_of_ethylene_receptor_ETR1isotropicsample_condition_1
3D HNCAsample_Receiver_domain_of_ethylene_receptor_ETR1isotropicsample_condition_1
3D HN(CO)CAsample_Receiver_domain_of_ethylene_receptor_ETR1isotropicsample_condition_1
3D HNCACBsample_Receiver_domain_of_ethylene_receptor_ETR1isotropicsample_condition_1
3D HN(COCA)CBsample_Receiver_domain_of_ethylene_receptor_ETR1isotropicsample_condition_1
3D HNCOsample_Receiver_domain_of_ethylene_receptor_ETR1isotropicsample_condition_1
3D HBHA(CO)NHsample_Receiver_domain_of_ethylene_receptor_ETR1isotropicsample_condition_1
3D H(CCO)NHsample_Receiver_domain_of_ethylene_receptor_ETR1isotropicsample_condition_1
3D C(CO)NHsample_Receiver_domain_of_ethylene_receptor_ETR1isotropicsample_condition_1
3D HCCH-COSYsample_Receiver_domain_of_ethylene_receptor_ETR1isotropicsample_condition_1
3D HCCH-TOCSYsample_Receiver_domain_of_ethylene_receptor_ETR1isotropicsample_condition_1
3D 1H-15N NOESYsample_Receiver_domain_of_ethylene_receptor_ETR1isotropicsample_condition_1
3D 1H-13C NOESY aliphaticsample_Receiver_domain_of_ethylene_receptor_ETR1isotropicsample_condition_1
3D 1H-13C NOESY aromaticsample_Receiver_domain_of_ethylene_receptor_ETR1isotropicsample_condition_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

Topspin, Bruker Biospin - collection

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

PDB
GB AAA70047 AAG52169 AAO47024 AAO47025 AAO47026
REF NP_176808
SP P49333

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts