BMRB Entry 19686

Title:
ASD-1 RRM domain bound to UGCAUGG RNA
Deposition date:
2013-12-15
Original release date:
2014-08-29
Authors:
Amrane, Samir; Mackereth, Cameron
Citation:

Citation: Amrane, Samir; Rebora, Karine; Zniber, Ilyass; Dupuy, Denis; Mackereth, Cameron. "BACKBONE-INDEPENDENT NUCLEIC ACID BINDING BY SPLICING FACTOR SUP-12 REVEALS KEY ASPECTS OF MOLECULAR RECOGNITION"  Nat. Commun. ., .-. (2014).

Assembly members:

Assembly members:
ASD-1, polymer, 99 residues, Formula weight is not available
UGCAUGG, polymer, 7 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: nematodes   Taxonomy ID: 6239   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Caenorhabditis elegans

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETHis1a

Data sets:
Data typeCount
13C chemical shifts185
15N chemical shifts87
1H chemical shifts87

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ASD-11
2RNA2

Entities:

Entity 1, ASD-1 99 residues - Formula weight is not available

The N-terminal Gly-Ala-Met remains following removal of the His6-tag by TEV potease

1   GLYALAMETGLYPROVALALAPROASNASP
2   ARGSERTHRSERSERSERSERTHRASPGLY
3   PROARGARGLEUHISVALSERASNILEPRO
4   PHELYSTYRARGGLUPROASPLEUTHRALA
5   METPHEGLULYSVALGLYPROVALVALASP
6   VALGLUILEILEPHEASNGLUARGGLYSER
7   LYSGLYPHEGLYPHEVALTHRMETGLNASN
8   PROASPASPALAASPARGALAARGALAGLU
9   PHEASNGLYTHRTHRILEGLUGLYARGARG
10   VALGLUVALASNLEUALATHRGLNARG

Entity 2, RNA 7 residues - Formula weight is not available

1   UGCAUGG

Samples:

sample_1: ASD-1, [U-99% 13C; U-99% 15N], 0.2 mM; sodium phosphate 20 mM; sodium chloride 300 mM; D2O, [U-2H], 10%; UGCAUGG 0.25 mM; H2O 90%

sample_conditions_1: ionic strength: 320 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

TOPSPIN v2.1, Bruker Biospin - collection, processing

NMRPipe v2007, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing, processing

SPARKY v3.113, Goddard - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Bruker Avance 700 MHz

Related Database Links:

UNP G5EEW7 .
BMRB 19609 19680
PDB
EMBL CAA85276 CAD82915 CCU83325
GB ABF22491
REF NP_001293646 NP_497841 NP_871667
AlphaFold G5EEW7

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks