BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 19568

Title: assignment of the transmembrane domain of insulin receptor in detergent micelles   PubMed: 24440425

Authors: Li, Qingxin; Wong, Yinglei; Kang, Congbao

Citation: Li, Qingxin; Wong, Ying Lei; Kang, CongBao. "Solution structure of the transmembrane domain of the insulin receptor in detergent micelles."  Biochim. Biophys. Acta 1838, 1313-1321 (2014).

Assembly members:
ISU, polymer, 57 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
ISU: MTYFYVTDYLDVPSNIAKII IGPLIFVFLFSVVIGSIYLF LRKRQPDGPLEHHHHHH

Data sets:
Data typeCount
13C chemical shifts136
15N chemical shifts46
1H chemical shifts98

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ISUR1

Entities:

Entity 1, ISUR 57 residues - Formula weight is not available

1   METTHRTYRPHETYRVALTHRASPTYRLEU
2   ASPVALPROSERASNILEALALYSILEILE
3   ILEGLYPROLEUILEPHEVALPHELEUPHE
4   SERVALVALILEGLYSERILETYRLEUPHE
5   LEUARGLYSARGGLNPROASPGLYPROLEU
6   GLUHISHISHISHISHISHIS

Samples:

sample_1: ISU, [U-100% 15N], 0.2 – 0.4 mM; ISU, [U-100% 13C; U-100% 15N], 0.2 – 0.4 mM; sodium phosphate 20 mM; DPC 2%; DTT 1 mM

sample_conditions_1: ionic strength: 20 mM; pH: 6.5; pressure: 1 atm; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

TOPSPIN v2.1, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView v4.1, Johnson, One Moon Scientific - data analysis

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 700 MHz

Related Database Links:

PDB
DBJ BAD92098
EMBL CAA26096
GB AAA39318 AAA41441 AAA59174 AAA59176 AAA59177
PRF 1104300A
REF NP_000199 NP_001073285 NP_034698 NP_058767 XP_001094337
SP P06213 P15127 P15208
TPG DAA27957