BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 19561

Title: 1H, 13C and 15N backbone NMR assignments of human Taf15-RRM-RanBP   PubMed: 24659459

Authors: Kashyap, Maruthi; Ganguly, Akshay Kumar; Bhavesh, Neel Sarovar

Citation: Kashyap, Maruthi; Ganguly, Akshay Kumar; Bhavesh, Neel Sarovar. "Sequence-specific resonance assignments of human TAF15-RRM and TAF15-RRM-RanBP2."  Biomol. NMR Assignments ., .-. (2014).

Assembly members:
Taf-RRM-RanBP, polymer, 162 residues, Formula weight is not available

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Taf-RRM-RanBP: GSHMSDNNTIFVQGLGEGVS TDQVGEFFKQIGIIKTNKKT GKPMINLYTDKDTGKPKGEA TVSFDDPPSAKAAIDWFDGK EFHGNIIKVSFATRRPEFMR GGGSGGGRRGRGGYRGRGGF QGRGGDPKSGDWVCPNPSCG NMNFARRNSCNQCNEPRPED SR

Data sets:
Data typeCount
13C chemical shifts414
15N chemical shifts146
1H chemical shifts146

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Taf-RRM-RanBP1

Entities:

Entity 1, Taf-RRM-RanBP 162 residues - Formula weight is not available

1   GLYSERHISMETSERASPASNASNTHRILE
2   PHEVALGLNGLYLEUGLYGLUGLYVALSER
3   THRASPGLNVALGLYGLUPHEPHELYSGLN
4   ILEGLYILEILELYSTHRASNLYSLYSTHR
5   GLYLYSPROMETILEASNLEUTYRTHRASP
6   LYSASPTHRGLYLYSPROLYSGLYGLUALA
7   THRVALSERPHEASPASPPROPROSERALA
8   LYSALAALAILEASPTRPPHEASPGLYLYS
9   GLUPHEHISGLYASNILEILELYSVALSER
10   PHEALATHRARGARGPROGLUPHEMETARG
11   GLYGLYGLYSERGLYGLYGLYARGARGGLY
12   ARGGLYGLYTYRARGGLYARGGLYGLYPHE
13   GLNGLYARGGLYGLYASPPROLYSSERGLY
14   ASPTRPVALCYSPROASNPROSERCYSGLY
15   ASNMETASNPHEALAARGARGASNSERCYS
16   ASNGLNCYSASNGLUPROARGPROGLUASP
17   SERARG

Samples:

sample_1: Taf-RRM-RanBP, [U-13C; U-15N; U-2H], 1 mM; H2O 90%; D2O, [U-100% 2H], 10%; Na2SO4 50 mM; 2-mercaptoethanol 5 mM; phosphate buffer 20 mM

sample_conditions_1: ionic strength: 0.05 M; pH: 6; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

NMR spectrometers:

  • Bruker Avance 700 MHz

Related Database Links:

BMRB 19320
PDB
DBJ BAA33811 BAA33812 BAB46889 BAC34676 BAE27086
EMBL CAA67398
GB AAC50932 AAH46099 AAI37592 AAI66769 AAO13485
REF NP_001099294 NP_001302693 NP_003478 NP_081703 NP_631961
SP Q92804
TPG DAA19087