BMRB Entry 19533

Title:
Solution Structure of NusE (S10) from Thermotoga maritima
Deposition date:
2013-10-01
Original release date:
2013-11-04
Authors:
Droegemueller, Johanna; Schweimer, Kristian; Roesch, Paul
Citation:

Citation: Droegemueller, Johanna; Schweimer, Kristian; Roesch, Paul. "Solution Structure of NusE from Thermotoga maritima"  .

Assembly members:

Assembly members:
entity, polymer, 82 residues, 9043.727 Da.

Natural source:

Natural source:   Common Name: Thermotoga maritima   Taxonomy ID: 2336   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Thermotoga maritima

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET29b

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts316
15N chemical shifts68
1H chemical shifts516

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1NusE (S10) from Thermotoga maritima1

Entities:

Entity 1, NusE (S10) from Thermotoga maritima 82 residues - 9043.727 Da.

construct used for structure determination: Ser1-Met2:Glu46-Ser47-Arg88_Leu88 Ser1 (cloning artefact from protease cleveage site) Met2:Glu46 (corresponds to Met1:Glu45 of wild type sequence Ser47 (replacement of ribosomal binding loop) Arg48:Leu82 (corresponds to Arg68:Leu102 of wild type sequence)

1   SERMETGLYGLYGLNLYSILEARGILELYS
2   LEULYSALATYRASPHISGLULEULEUASP
3   GLUSERALALYSLYSILEVALGLUVALALA
4   LYSSERTHRASNSERLYSVALSERGLYPRO
5   ILEPROLEUPROTHRGLUSERARGVALHIS
6   LYSARGLEUILEASPILEILEASPPROSER
7   PROLYSTHRILEASPALALEUMETARGILE
8   ASNLEUPROALAGLYVALASPVALGLUILE
9   LYSLEU

Samples:

sample_1: entity, [U-99% 13C; U-99% 15N], 0.9 mM; HEPES 25 mM; sodium chloride 50 mM

sample_conditions_1: pH: 7.5; pressure: 1 atm; temperature: 323 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D CCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1

Software:

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMRView, Johnson, One Moon Scientific - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 600 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks