BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 19406

Title: K11-linked Diubiquitin average solution structure at pH 6.8, 0 mM NaCl   PubMed: 23823328

Authors: Castaneda, Carlos; Fushman, David

Citation: Castaneda, Carlos; Kashyap, Tanuja; Nakasone, Mark; Krueger, Susan; Fushman, David. "Unique structural, dynamical, and functional properties of K11-linked polyubiquitin chains"  Structure 21, 1168-1181 (2013).

Assembly members:
entity, polymer, 152 residues, 8576.914 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
entity: MQIFVKTLTGKTITLEVEPS DTIENVKAKIQDKEGIPPDQ QRLIFAGKQLEDGRTLSDYN IQKESTLHLVLRLRGGMQIF VKTLTGKTITLEVEPSDTIE NVKAKIQDKEGIPPDQQRLI FAGKQLEDGRTLSDYNIQKE STLHLVLRLRGG

Data sets:
Data typeCount
15N chemical shifts140
1H chemical shifts140

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1K11-linked Diubiquitin_11
2K11-linked Diubiquitin_21

Entities:

Entity 1, K11-linked Diubiquitin_1 152 residues - 8576.914 Da.

1   METGLNILEPHEVALLYSTHRLEUTHRGLY
2   LYSTHRILETHRLEUGLUVALGLUPROSER
3   ASPTHRILEGLUASNVALLYSALALYSILE
4   GLNASPLYSGLUGLYILEPROPROASPGLN
5   GLNARGLEUILEPHEALAGLYLYSGLNLEU
6   GLUASPGLYARGTHRLEUSERASPTYRASN
7   ILEGLNLYSGLUSERTHRLEUHISLEUVAL
8   LEUARGLEUARGGLYGLYMETGLNILEPHE
9   VALLYSTHRLEUTHRGLYLYSTHRILETHR
10   LEUGLUVALGLUPROSERASPTHRILEGLU
11   ASNVALLYSALALYSILEGLNASPLYSGLU
12   GLYILEPROPROASPGLNGLNARGLEUILE
13   PHEALAGLYLYSGLNLEUGLUASPGLYARG
14   THRLEUSERASPTYRASNILEGLNLYSGLU
15   SERTHRLEUHISLEUVALLEUARGLEUARG
16   GLYGLY

Samples:

sample_1: entity, [U-99% 15N], 75 uM; sodium phosphate 20 mM; H2O 93%; D2O 7%

sample_conditions_1: pH: 6.8; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N IPAP HSQCsample_1anisotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

SPARKY, Bruker Biospin, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Goddard - collection, data analysis, processing

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

BMRB 11505 11547 15047 15410 15907 16228 16582 16626 17181 17439 17769 17919 18582 18583 18584 18610 18611 18737 19412 25070 25601 26604 4245 4375
PDB
DBJ BAA03983 BAA09096 BAA09860 BAA11842 BAA11843
EMBL CAA25706 CAA26488 CAA28495 CAA30183 CAA30815
GB AAA28154 AAA28997 AAA28998 AAA28999 AAA29000
PIR I50437 I51568 I65237 JN0790 JT0492
PRF 1101405A 1212243A 1212243C 1212243F 1212243G
REF NP_001005123 NP_001006688 NP_001009117 NP_001009202 NP_001009286
SP P0C273 P0C275 P0C276 P0CG47 P0CG48
TPD FAA00319
TPE CEL68433 CEL70397 CEL75964 CEL78064
TPG DAA18802 DAA20663 DAA20672 DAA24675 DAA28295