BMRB Entry 19386

Title:
parallel-stranded G-quadruplex in DNA poly-G stretches
Deposition date:
2013-07-24
Original release date:
2014-12-01
Authors:
Sengar, Anjali; Heddi, Brahim; Phan, Anh Tuan
Citation:

Citation: Sengar, Anjali; Heddi, Brahim; Phan, Anh Tuan. "Formation of G-quadruplexes in poly-G sequences: Structure of a propeller-type parallel-stranded G-quadruplex formed by a G15 stretch"  Biochemistry ., .-. (2014).
PubMed: 25375976

Assembly members:

Assembly members:
DNA_(5'-D(*TP*TP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*T)-3'), polymer, 18 residues, 5805.756 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
DNA_(5'-D(*TP*TP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*T)-3'): TTGGGGGGGGGGGGGGGT

Data sets:
Data typeCount
1H chemical shifts164

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DNA (5'-D(*TP*TP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*T)-3')1

Entities:

Entity 1, DNA (5'-D(*TP*TP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*T)-3') 18 residues - 5805.756 Da.

1   DTDTDGDGDGDGDGDGDGDG
2   DGDGDGDGDGDGDGDT

Samples:

sample_1: DNA (5'-D(*TP*TP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*T)-3')0.1 – 2 mM; H2O 90%; D2O 10%; KCl 5 mM; KPi 5 mM

sample_conditions_1: ionic strength: 10 mM; pH: 7.0; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H JRNOESYsample_1isotropicsample_conditions_1

Software:

X-PLOR NIH v2.34, Schwieters, Kuszewski, Tjandra and Clore - geometry optimization, refinement, structure solution

NMR spectrometers:

  • Bruker AMX 700 MHz
  • Bruker AMX 600 MHz