BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 19366

Title: NMR structure of region 2 of E. coli sigmaE   PubMed: 24531660

Authors: Campagne, Sebastien; Vorholt, Julia; Allain, Frederic

Citation: Campagne, Sebastien; Marsh, May; Capitani, Guido; Vorholt, Julia; Allain, Frederic H-T. "Structural basis for -10 promoter element melting by environmentally induced sigma factors."  Nat. Struct. Mol. Biol. 21, 269-276 (2014).

Assembly members:
entity, polymer, 106 residues, 12252.029 Da.

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
entity: MSEQLTDQVLVERVQKGDQK AFNLLVVRYQHKVASLVSRY VPSGDVPDVVQEAFIKAYRA LDSFRGDSAFYTWLYRIAVN TAKNYLVAQGRRLELVPRGS HHHHHH

Data sets:
Data typeCount
13C chemical shifts334
15N chemical shifts108
1H chemical shifts696

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1region 2 of E. coli sigmaE1

Entities:

Entity 1, region 2 of E. coli sigmaE 106 residues - 12252.029 Da.

1   METSERGLUGLNLEUTHRASPGLNVALLEU
2   VALGLUARGVALGLNLYSGLYASPGLNLYS
3   ALAPHEASNLEULEUVALVALARGTYRGLN
4   HISLYSVALALASERLEUVALSERARGTYR
5   VALPROSERGLYASPVALPROASPVALVAL
6   GLNGLUALAPHEILELYSALATYRARGALA
7   LEUASPSERPHEARGGLYASPSERALAPHE
8   TYRTHRTRPLEUTYRARGILEALAVALASN
9   THRALALYSASNTYRLEUVALALAGLNGLY
10   ARGARGLEUGLULEUVALPROARGGLYSER
11   HISHISHISHISHISHIS

Samples:

sample_1: entity, [U-100% 13C; U-100% 15N], 0.5 mM; H2O 90%; D2O 10%; NaPO4 10 mM; NaCl 50 mM

sample_conditions_1: ionic strength: 0.07 M; pH: 6.5; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1

Software:

AMBER v9, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollman - refinement

NMR spectrometers:

  • Bruker Avance 900 MHz
  • Bruker Avance 500 MHz

Related Database Links:

BMRB 19367
PDB
DBJ BAA10920 BAB36862 BAE76749 BAG78385 BAI26818
EMBL CAD02789 CAP77019 CAQ32948 CAQ88049 CAQ99523
GB AAA57186 AAC45314 AAC75626 AAG57689 AAL21534
PIR AI0829
REF NP_311466 NP_417068 NP_457116 NP_461575 NP_708425
SP D0ZSY9 P0A2F0 P0A2F1 P0AGB6 P0AGB7