BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 19334

Title: NMR structure of Temporin-1 Ta in lipopolysaccharide micelles: Mechanistic insight into inactivation by outer memebrane   PubMed: 24039798

Authors: Mohanram, Harini

Citation: Saravanan, Rathi; Joshi, Mangesh; Mohanram, Harini; Bhunia, Anirban; Mangoni, Maria Luisa; Bhattacharjya, Surajit. "NMR structure of temporin-1 ta in lipopolysaccharide micelles: mechanistic insight into inactivation by outer membrane."  PLoS ONE 8, .-. (2013).

Assembly members:
entity, polymer, 13 residues, 1398.770 Da.

Natural source:   Common Name: Bullfrog   Taxonomy ID: 8400   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rana catesbeiana

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
entity: FLPLIGRVLSGIL

Data sets:
Data typeCount
1H chemical shifts76

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Temporin-1 Ta in lipopolysaccharide micelles1

Entities:

Entity 1, Temporin-1 Ta in lipopolysaccharide micelles 13 residues - 1398.770 Da.

1   PHELEUPROLEUILEGLYARGVALLEUSER
2   GLYILELEU

Samples:

sample_1: entity; H2O 90 mM; D2O 10 mM

sample_conditions_1: ionic strength: 55 mM; pH: 4.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker DRX 600 MHz

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