BMRB Entry 19312

Title:
Solution NMR Structure of Transcription Factor GATA-4 from Homo sapiens, Northeast Structural Genomics Consortium (NESG) Target HR4783B
Deposition date:
2013-06-20
Original release date:
2013-07-08
Authors:
Xu, Xianzhong; Eletsky, Alexander; Lee, Dan; Kohn, Eitan; Janjua, Haleema; Xiao, Rong; Acton, Thomas; Everett, John; Montelione, Gaetano; Szyperski, Thomas
Citation:

Citation: Xu, Xianzhong; Eletsky, Alexander; Lee, Dan; Kohn, Eitan; Janjua, Haleema; Xiao, Rong; Acton, Thomas; Everett, John; Montelione, Gaetano; Szyperski, Thomas. "Solution NMR Structure of a Transcription Factor GATA-4 from Homo sapiens, Northeast Structural Genomics Consortium (NESG) Target HR4783B"  To be published ., .-..

Assembly members:

Assembly members:
HR4783B, polymer, 63 residues, 7120.694 Da.
ZINC ION, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15Nano6HT_NESG

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts253
15N chemical shifts62
1H chemical shifts395

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HR4783B1
2ZINC ION2

Entities:

Entity 1, HR4783B 63 residues - 7120.694 Da.

Residues 1-3 represent the remained purification His tag after cleavage.

1   SERHISMETSERALASERARGARGVALGLY
2   LEUSERCYSALAASNCYSGLNTHRTHRTHR
3   THRTHRLEUTRPARGARGASNALAGLUGLY
4   GLUPROVALCYSASNALACYSGLYLEUTYR
5   METLYSLEUHISGLYVALPROARGPROLEU
6   ALAMETARGLYSGLUGLYILEGLNTHRARG
7   LYSARGLYS

Entity 2, ZINC ION - Zn - 65.409 Da.

1   ZN

Samples:

hr4783b.009: HR4783B.009, [U-100% 13C; U-100% 15N], 0.378 mM; sodium chloride 100 mM; DTT 5 mM; Tris-HCl 10 mM; DSS 50 uM; sodium azide 0..02%; ZINC ION 50 uM; H2O 90%; D2O 10%

hr4783b.005: HR4783B.009, [U-5% 13C; U-100% 15N], 0.156 mM; sodium chloride 100 mM; DTT 5 mM; Tris-HCl 10 mM; DSS 50 uM; sodium azide 0..02%; ZINC ION 50 uM; H2O 90%; D2O 10%

sample_conditions_1: pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQChr4783b.009isotropicsample_conditions_1
2D CT 1H-13C HSQC alihr4783b.009isotropicsample_conditions_1
3D HNCOhr4783b.009isotropicsample_conditions_1
3D CBCA(CO)NHhr4783b.009isotropicsample_conditions_1
3D HNCACBhr4783b.009isotropicsample_conditions_1
2D CT 1H-13C HSQC arohr4783b.009isotropicsample_conditions_1
3D simutaneous 13C-aromatic,13C-aliphatic,15N edited 1H-1H NOESYhr4783b.009isotropicsample_conditions_1
3D CCH-TOCSYhr4783b.009isotropicsample_conditions_1
3D HBHA(CO)NHhr4783b.009isotropicsample_conditions_1
2D 1H-15N HSQC_Hishr4783b.009isotropicsample_conditions_1
2D 1H-15N HSQC_widehr4783b.009isotropicsample_conditions_1
3D (H)CCH-COSY alihr4783b.009isotropicsample_conditions_1
2D 1H-13C HSQC_methylhr4783b.005isotropicsample_conditions_1

Software:

CNS v1.3, Brunger, Adams, Clore, Gros, Nilges and Read - refinemen,structure solution,geometry optimization

CYANA v3.0, Guntert, Mumenthaler and Wuthrich - refinement,geometry optimization,structure solution

AutoStruct v2.1, Huang, Tejero, Powers and Montelione - data analysis,refinement

AutoAssign v2.3.1, Zimmerman, Moseley, Kulikowski and Montelione - data analysis,chemical shift assignment

XEASY, Bartels et al. - data analysis,peak picking,chemical shift assignment

VNMRJ v2.3.1, Varian - collection

TALOS+, Shen, Cornilescu, Delaglio and Bax - geometry optimization

PSVS v1.4, Bhattacharya, Montelione - structure validation

PROSA, Guntert - data analysis

CSI, (CSI)-Wishart and Sykes - data analysis

CARA v1.8.4, (CARA)-Keller and Wuthrich - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Varian INOVA 750 MHz

Related Database Links:

PDB
GB ABG02399 ABM67327 AGC95106 EHH28277 EHH63983
REF XP_005337311 XP_012962956

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks