BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 19284

Title: Relaxation data of the delta subunit of RNA polymerase from Bacillus subtilis   PubMed: 23868186

Authors: Papouskova, Veronika; Zidek, Lukas; Kaderavek, Pavel; Sanderova, Hana; Krasny, Libor; Sklenar, Vladimir

Citation: Papoukova, Veronika; Kadeavek, Pavel; Otrusinova, Olga; Rabatinova, Albeta; Sanderova, Hana; Novaek, Jii; Krasny, Libor; Sklena, Vladimir; Zidek, Luka. "Structural Study of the Partially Disordered Full-Length Subunit of RNA Polymerase from Bacillus subtilis."  Chembiochem 14, 1772-1779 (2013).

Assembly members:
delta, polymer, 172 residues, Formula weight is not available

Natural source:   Common Name: Firmicutes   Taxonomy ID: 1423   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacillus subtilis

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
delta: GIKQYSQEELKEMALVEIAH ELFEEHKKPVPFQELLNEIA SLLGVKKEELGDRIAQFYTD LNIDGRFLALSDQTWGLRSW YPYDQLDEETQPTVKAKKKK AKKAVEEDLDLDEFEEIDED DLDLDEVEEELDLEADDFDE EDLDEDDDDLEIEEDIIDED DEDYDDEEEEIK

Data sets:
Data typeCount
heteronuclear NOE values103
T1 relaxation values105
T2 relaxation values105

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1delta1

Entities:

Entity 1, delta 172 residues - Formula weight is not available

1   GLYILELYSGLNTYRSERGLNGLUGLULEU
2   LYSGLUMETALALEUVALGLUILEALAHIS
3   GLULEUPHEGLUGLUHISLYSLYSPROVAL
4   PROPHEGLNGLULEULEUASNGLUILEALA
5   SERLEULEUGLYVALLYSLYSGLUGLULEU
6   GLYASPARGILEALAGLNPHETYRTHRASP
7   LEUASNILEASPGLYARGPHELEUALALEU
8   SERASPGLNTHRTRPGLYLEUARGSERTRP
9   TYRPROTYRASPGLNLEUASPGLUGLUTHR
10   GLNPROTHRVALLYSALALYSLYSLYSLYS
11   ALALYSLYSALAVALGLUGLUASPLEUASP
12   LEUASPGLUPHEGLUGLUILEASPGLUASP
13   ASPLEUASPLEUASPGLUVALGLUGLUGLU
14   LEUASPLEUGLUALAASPASPPHEASPGLU
15   GLUASPLEUASPGLUASPASPASPASPLEU
16   GLUILEGLUGLUASPILEILEASPGLUASP
17   ASPGLUASPTYRASPASPGLUGLUGLUGLU
18   ILELYS

Samples:

sample_1: delta, [U-100% 15N], 1.1 mM; D2O, [U-99% 2H], 10%; sodium chloride 10 mM; sodium azide 0.05%; phosphate buffer 20 mM; H2O 90%

sample_conditions_1: ionic strength: 20 mM; pH: 6.6; pressure: 1 atm; temperature: 301 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D NOE 15N,1H correlationsample_1isotropicsample_conditions_1
2D R1 15N,1H correlationsample_1isotropicsample_conditions_1
2D R2 15N,1H correlationsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment, data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

BMRB 16635 16912 18903
PDB
DBJ BAM55799 BAM59811
EMBL CAA89869 CAB15744 CCU60814 CEI59524 CEJ79381
GB AAA22710 ADM39706 ADV94526 AEP92774 AFI30331
REF NP_391597 WP_003221959 WP_003227609 WP_003235832 WP_003243661
SP P12464