BMRB Entry 19283

Title:
Solution NMR structure of the RXFP2 LDLa module
Deposition date:
2013-06-03
Original release date:
2014-06-02
Authors:
Petrie, Emma; Gooley, Paul; Bathgate, Ross
Citation:

Citation: Petrie, Emma; Gooley, Paul; Bathgate, Ross. "Solution NMR structure of the RXFP2 LDLa module"  To be Published ., .-..

Assembly members:

Assembly members:
RXFP2_LDLa, polymer, 44 residues, 4646.191 Da.
CALCIUM ION, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEV2_LDL8

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RXFP2_LDLa: GSMITPSCQKGYFPCGNLTK CLPRAFHCDGKDDCGNGADE ENCG

Data sets:
Data typeCount
13C chemical shifts107
15N chemical shifts43
1H chemical shifts254

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RXFP2_LDLa1
2CALCIUM ION2

Entities:

Entity 1, RXFP2_LDLa 44 residues - 4646.191 Da.

1   GLYSERMETILETHRPROSERCYSGLNLYS
2   GLYTYRPHEPROCYSGLYASNLEUTHRLYS
3   CYSLEUPROARGALAPHEHISCYSASPGLY
4   LYSASPASPCYSGLYASNGLYALAASPGLU
5   GLUASNCYSGLY

Entity 2, CALCIUM ION - 40.078 Da.

1   CA

Samples:

sample_1: RXFP2_LDLa, [U-99% 15N], 2.0 mM; imidazole 50 mM; CALCIUM ION 10 mM; H2O 90%; D2O 10%

sample_2: RXFP2_LDLa, [U-99% 13C; U-99% 15N], 2.5 mM; imidazole 50 mM; CALCIUM ION 10 mM; H2O 90%; D2O 10%

sample_3: RXFP2_LDLa 0.5 mM; imidazole, [U-2H], 50 mM; CALCIUM ION 10 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0 M; pH: 5.5; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_2isotropicsample_conditions_1
3D C(CO)NHsample_2isotropicsample_conditions_1
3D 1H-13C NOESYsample_2isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNHBsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_3isotropicsample_conditions_1
2D 1H-1H TOCSYsample_3isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CcpNMR, CCPN - chemical shift assignment

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

PDB
DBJ BAI45372
GB AAI46280 AAI48730 AAL69324 AAL73946 AAX85199
REF NP_001159530 NP_570718 XP_002824193 XP_002824194 XP_003826911
SP Q8WXD0
AlphaFold Q8WXD0

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks