BMRB Entry 19255

Title:
Backbone amide chemical shifts and relaxation data (T1-T2) for the Y48A mutant of the FimH adhesin carbohydrate-binding domain
Deposition date:
2013-05-22
Original release date:
2014-02-13
Authors:
Vanwetswinkel, Sophie; Van Nuland, N.
Citation:

Citation: Vanwetswinkel, Sophie; Volkov, Alexander; Sterckx, Yann; Garcia-Pino, Abel; Buts, Lieven; Vranken, Wim; Bouckaert, Julie; Roy, Rene; Wyns, Lode; van Nuland, Nico. "Study of the Structural and Dynamic Effects in the FimH Adhesin upon alfa-D-Heptyl Mannose Binding"  J. Med. Chem. 57, 1416-1427 (2014).
PubMed: 24476493

Assembly members:

Assembly members:
FIMH_Y48A_mutant, polymer, 158 residues, 16904.8881 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET24a

Data sets:
Data typeCount
15N chemical shifts171
1H chemical shifts189
T1 relaxation values132
T2 relaxation values132

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1FIMH Y48A mutant1

Entities:

Entity 1, FIMH Y48A mutant 158 residues - 16904.8881 Da.

1   PHEALACYSLYSTHRALAASNGLYTHRALA
2   ILEPROILEGLYGLYGLYSERALAASNVAL
3   TYRVALASNLEUALAPROVALVALASNVAL
4   GLYGLNASNLEUVALVALASPLEUSERTHR
5   GLNILEPHECYSHISASNASPALAPROGLU
6   THRILETHRASPTYRVALTHRLEUGLNARG
7   GLYSERALATYRGLYGLYVALLEUSERASN
8   PHESERGLYTHRVALLYSTYRSERGLYSER
9   SERTYRPROPHEPROTHRTHRSERGLUTHR
10   PROARGVALVALTYRASNSERARGTHRASP
11   LYSPROTRPPROVALALALEUTYRLEUTHR
12   PROVALSERSERALAGLYGLYVALALAILE
13   LYSALAGLYSERLEUILEALAVALLEUILE
14   LEUARGGLNTHRASNASNTYRASNSERASP
15   ASPPHEGLNPHEVALTRPASNILETYRALA
16   ASNASNASPVALVALVALPROTHR

Samples:

sample_1: FIMH Y48A mutant, [U-100% 15N], 0.0011 M; sodium phosphate 0.02 M; sodium chloride 0.1 M; H2O 93%; D2O 7%

sample_conditions_1: ionic strength: 0.12 M; pH: 6.000; pressure: 1.000 atm; temperature: 298.000 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1solutionsample_conditions_1
3D 1H-15N NOESYsample_1solutionsample_conditions_1
2D 1H-15N HSQC (inverse recovery)sample_1solutionsample_conditions_1
2D 1H-15N HSQC (CPMG)sample_1solutionsample_conditions_1

Software:

ANALYSIS v2.1 -

NMRDraw vany -

NMRPipe vany -

NMR spectrometers:

  • Varian Direct-Drive 600 MHz

Related Database Links:

UNP A2IC68_ECOLX
AlphaFold A2IC68

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks