BMRB Entry 19239

Title:
2C TCR
Deposition date:
2013-05-14
Original release date:
2013-07-23
Authors:
Hawse, Bill; Baker, Brian
Citation:

Citation: Hawse, WIlliam; Baker, Brian. "Assigned Chemical Shifts for the mini 2C TCR"  .

Assembly members:

Assembly members:
2c_TCR, polymer, 248 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet28a

Data sets:
Data typeCount
13C chemical shifts543
15N chemical shifts188
1H chemical shifts188

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
12c_TCR1

Entities:

Entity 1, 2c_TCR 248 residues - Formula weight is not available

1   GLUALAALAVALTHRGLNSERPROARGASN
2   LYSVALALAVALTHRGLYGLULYSVALTHR
3   LEUSERCYSASNGLNTHRASNASNHISASN
4   ASNMETTYRTRPTYRARGGLNASPTHRGLY
5   HISGLULEUARGLEUILEHISTYRSERTYR
6   GLYALAGLYSERTHRGLULYSGLYASPILE
7   PROASPGLYTYRLYSALASERARGPROSER
8   GLNGLUASNPHESERLEUTHRLEUGLUSER
9   ALATHRPROSERGLNTHRSERVALTYRPHE
10   CYSALASERGLYGLYGLYGLYTHRLEUTYR
11   PHEGLYALAGLYTHRARGLEUSERVALLEU
12   SERSERALAASPASPALALYSLYSASPALA
13   ALALYSLYSASPASPALALYSLYSASPASP
14   ALALYSLYSASPALAGLNSERVALTHRGLN
15   PROASPALAARGVALTHRVALSERGLUGLY
16   ALASERLEUGLNLEUARGCYSLYSTYRSER
17   TYRSERALATHRPROTYRLEUPHETRPTYR
18   VALGLNTYRPROARGGLNGLYPROGLNLEU
19   LEULEULYSTYRTYRSERGLYASPPROVAL
20   VALGLNGLYVALASNGLYPHEGLUALAGLU
21   PHESERLYSSERASNSERSERPHEHISLEU
22   ARGLYSALASERVALHISARGSERASPSER
23   ALAVALTYRPHECYSALAVALSERGLYPHE
24   ALASERALALEUTHRPHEGLYSERGLYTHR
25   LYSVALILEVALLEUPROTYRASN

Samples:

sample_1: 2c TCR, [U-99% 13C; U-99% 15N], 0.6 mM; sodium chloride 10 mM; TRIS 20 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 50 mM; pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HCACOsample_1isotropicsample_conditions_1

Software:

Analysis, CCPN - data analysis

TOPSPIN, Bruker Biospin - data analysis

SPARKY, Goddard - data analysis

NMR spectrometers:

  • Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks