BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 19238

Title: Backbone 1H, 13C, 15N resonance assignments of calcium-bound calmodulin in complex with PSD95 N-terminal peptide   PubMed: 24705785

Authors: Zhang, Yonghong; Ames, James

Citation: Zhang, Yonghong; Matt, Lucas; Patriarchi, Tommaso; Malik, Zulfiqar; Chowdhury, Dhrubajyoti; Park, Deborah; Renieri, Alessandra; Ames, James; Hell, Johannes. "Capping of the N-terminus of PSD-95 by calmodulin triggers its postsynaptic release"  Embo J. 33, 1341-1353 (2014).

Assembly members:
Calmodulin_prototypical_calcium_sensor, polymer, 148 residues, Formula weight is not available
PSD95_N-terminal_peptide, polymer, 71 residues, Formula weight is not available

Natural source:   Common Name: African clawed frog   Taxonomy ID: 8355   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Xenopus laevis

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Calmodulin_prototypical_calcium_sensor: ADQLTEEQIAEFKEAFSLFD KDGDGTITTKELGTVMRSLG QNPTEAELQDMINEVDADGN GTIDFPEFLTMMARKMKDTD SEEEIREAFRVFDKDGNGYI SAAELRHVMTNLGEKLTDEE VDEMIREADIDGDGQVNYEE FVQMMTAK
PSD95_N-terminal_peptide: MDCLCIVTTKKYRYQDEDTP PLEHSPAHLPNQANSPPVIV NTDTLEAPGYELQVNGTEGE MEYEEITLERG

Data sets:
Data typeCount
13C chemical shifts440
15N chemical shifts143
1H chemical shifts581

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Calmodulin_prototypical_calcium_sensor1
2PSD95_N-terminal_peptide2

Entities:

Entity 1, Calmodulin_prototypical_calcium_sensor 148 residues - Formula weight is not available

1   ALAASPGLNLEUTHRGLUGLUGLNILEALA
2   GLUPHELYSGLUALAPHESERLEUPHEASP
3   LYSASPGLYASPGLYTHRILETHRTHRLYS
4   GLULEUGLYTHRVALMETARGSERLEUGLY
5   GLNASNPROTHRGLUALAGLULEUGLNASP
6   METILEASNGLUVALASPALAASPGLYASN
7   GLYTHRILEASPPHEPROGLUPHELEUTHR
8   METMETALAARGLYSMETLYSASPTHRASP
9   SERGLUGLUGLUILEARGGLUALAPHEARG
10   VALPHEASPLYSASPGLYASNGLYTYRILE
11   SERALAALAGLULEUARGHISVALMETTHR
12   ASNLEUGLYGLULYSLEUTHRASPGLUGLU
13   VALASPGLUMETILEARGGLUALAASPILE
14   ASPGLYASPGLYGLNVALASNTYRGLUGLU
15   PHEVALGLNMETMETTHRALALYS

Entity 2, PSD95_N-terminal_peptide 71 residues - Formula weight is not available

1   METASPCYSLEUCYSILEVALTHRTHRLYS
2   LYSTYRARGTYRGLNASPGLUASPTHRPRO
3   PROLEUGLUHISSERPROALAHISLEUPRO
4   ASNGLNALAASNSERPROPROVALILEVAL
5   ASNTHRASPTHRLEUGLUALAPROGLYTYR
6   GLULEUGLNVALASNGLYTHRGLUGLYGLU
7   METGLUTYRGLUGLUILETHRLEUGLUARG
8   GLY

Samples:

sample_1: Calmodulin, PSD95NT, DTT, Tris, CaCl2, [U-99% 13C; U-99% 15N], 400 ± 0.2 uM; PSD95_N-terminal_peptide 600 uM; Tris-d11 20 mM; NaCl 50 mM; CaCl2 5 mM; DTT-d 5 mM; H2O 93%; D2O 7%

sample_conditions_1: ionic strength: 0.1 M; pH: 7.0; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Goddard - peak picking, processing

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

BMRB 15184 15185 15186 15187 15188 15191 15470 15624 15650 15852 1634 16418 16465 1648 16764 17264 17360 17771 17807 18027 18028 18556 19036 19586 19604 25253 25257 26503 26626 26627 4056 4270 4284 4310
PDB
DBJ BAA08302 BAA11896 BAA19786 BAA19787 BAA19788 BAA09297 BAG10877 BAH11607
EMBL CAA10601 CAA32050 CAA32062 CAA32119 CAA32120 CAA47103
GB AAA35635 AAA35641 AAA37365 AAA40862 AAA40863 AAA41971 AAQ56705
PIR JC1305 MCON
PRF 0409298A 0608335A
REF NP_001008160 NP_001009759 NP_001027633 NP_001039714 NP_001040234 NP_031890 NP_062567 XP_004594887 XP_004638436 XP_004669318
SP O02367 O16305 O96081 P02594 P05932 P31016 P78352 Q62108
TPG DAA13808 DAA18029 DAA19590 DAA24777 DAA24988