BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 19216

Title: Structural and Functional Analysis of Transmembrane Segment IV of the Salt Tolerance Protein Sod2   PubMed: 23836910

Authors: Ullah, Asad; Kemp, Grant; Lee, Brian; Alves, Claudia; Young, Howard; Sykes, Brian; Fliegel, Larry

Citation: Ullah, Asad; Kemp, Grant; Lee, Brian; Alves, Claudia; Young, Howard; Sykes, Brian; Fliegel, Larry. "Structural and Functional Analysis of Transmembrane Segment IV of the Salt Tolerance Protein Sod2."  J. Biol. Chem. 288, 24609-24624 (2013).

Assembly members:
sod2_TM_IV, polymer, 38 residues, 3073.673 Da.

Natural source:   Common Name: Fission yeast   Taxonomy ID: 4896   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Schizosaccharomyces pombe

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
sod2_TM_IV: GSKKKLFPQINFLGSLLIAG CITSTDPVLSALIVGKKK

Data sets:
Data typeCount
15N chemical shifts35
1H chemical shifts176

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1sod2_TM_IV1

Entities:

Entity 1, sod2_TM_IV 38 residues - 3073.673 Da.

1   GLYSERLYSLYSLYSLEUPHEPROGLNILE
2   ASNPHELEUGLYSERLEULEUILEALAGLY
3   CYSILETHRSERTHRASPPROVALLEUSER
4   ALALEUILEVALGLYLYSLYSLYS

Samples:

sample_1: sod2_TM_IV, [U-99% 15N], 0.2 – 0.7 mM; CDCl3 50 v/v; 2-propanol 50 v/v

sample_conditions_1: pressure: 1 atm; temperature: 303.15 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N NOESY-HSQCsample_1isotropicsample_conditions_1
3D 1H-15N TOCSY-HSQCsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1

Software:

VNMRJ, Varian - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRViewJ, Johnson, One Moon Scientific - data analysis, peak picking

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMR spectrometers:

  • Varian INOVA 500 MHz

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