BMRB Entry 19172

Title:
PTPN11 C-SH2 domain free form
Deposition date:
2013-04-17
Original release date:
2013-08-15
Authors:
Rubio, Lucia; Huculeci, Radu; Buts, Lieven; Vanwetswinkel, Sophie; Lenaerts, Tom; van Nuland, Nico
Citation:

Citation: Rubio, Lucia; Huculeci, Radu; Buts, Lieven; Vanwetswinkel, Sophie; Lenaerts, Tom; van Nuland, Nico. "(1)H, (13)C, and (15)N backbone and side-chain chemical shift assignments of the free and bound forms of the human PTPN11 second SH2 domain."  Biomol. NMR Assignments ., .-. (2013).
PubMed: 23838815

Assembly members:

Assembly members:
PTPN11_C-SH2_free, polymer, 118 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15b

Data sets:
Data typeCount
13C chemical shifts507
15N chemical shifts120
1H chemical shifts805

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1C-SH21

Entities:

Entity 1, C-SH2 118 residues - Formula weight is not available

1   GLYSERHISMETTHRSERGLUARGTRPPHE
2   HISGLYHISLEUSERGLYLYSGLUALAGLU
3   LYSLEULEUTHRGLULYSGLYLYSHISGLY
4   SERPHELEUVALARGGLUSERGLNSERHIS
5   PROGLYASPPHEVALLEUSERVALARGTHR
6   GLYASPASPLYSGLYGLUSERASNASPGLY
7   LYSSERLYSVALTHRHISVALMETILEARG
8   CYSGLNGLULEULYSTYRASPVALGLYGLY
9   GLYGLUARGPHEASPSERLEUTHRASPLEU
10   VALGLUHISTYRLYSLYSASNPROMETVAL
11   GLUTHRLEUGLYTHRVALLEUGLNLEULYS
12   GLNPROLEUASNTHRTHRARGILE

Samples:

sample_1: PTPN11 C-SH2 free, [U-98% 13C; U-98% 15N], 0.7 – 1 mM; D2O 7%; H2O 93%

sample_conditions_1: ionic strength: 0 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
HBCBCGCDHDsample_1isotropicsample_conditions_1
HBCBCGCDCEHEsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CcpNMR, CCPN - chemical shift assignment

NMR spectrometers:

  • Varian VNS 600 MHz
  • Varian VNS 800 MHz

Related Database Links:

BMRB 19173
PDB
DBJ BAE37500
GB ACE60554
REF XP_004709716 XP_009480670 XP_010826895 XP_012878594 XP_013359171

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks