BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 19127

Title: The Dynamics of Lysozyme from Bacteriophage Lambda in Solution probed by NMR and MD simulations   PubMed: 23801644

Authors: Smith, Lorna; Bowen, Alice; DiPaolo, Alexandre; Matagne, Andre; Redfield, Christina

Citation: Smith, Lorna; Bowen, Alice; Di Paolo, Alexandre; Matagne, Andre; Redfield, Christina. "The dynamics of lysozyme from bacteriophage lambda in solution probed by NMR and MD simulations."  Chembiochem 14, 1780-1788 (2013).

Assembly members:
lambda_lysozyme, polymer, 158 residues, Formula weight is not available

Natural source:   Common Name: Bacteriophage lambda   Taxonomy ID: 10710   Superkingdom: Viruses   Kingdom: not available   Genus/species: Lambdalikevirus Enterobacteria phage lambda

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
lambda_lysozyme: MVEINNQRKAFLDMLAWSEG TDNGRQKTRNHGYDVIVGGE LFTDYSDHPRKLVTLNPKLK STGAGRYQLLSRWWDAYRKQ LGLKDFSPKSQDAVALQQIK ERGALPMIDRGDIRQAIDRC SNIWASLPGAGYGQFEHKAD SLIAKFKEAGGTVREIDV

Data sets:
Data typeCount
coupling constants104
heteronuclear NOE values256
residual dipolar couplings118
T1 relaxation values256
T2 relaxation values256

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1lambda_lysozyme1

Entities:

Entity 1, lambda_lysozyme 158 residues - Formula weight is not available

1   METVALGLUILEASNASNGLNARGLYSALA
2   PHELEUASPMETLEUALATRPSERGLUGLY
3   THRASPASNGLYARGGLNLYSTHRARGASN
4   HISGLYTYRASPVALILEVALGLYGLYGLU
5   LEUPHETHRASPTYRSERASPHISPROARG
6   LYSLEUVALTHRLEUASNPROLYSLEULYS
7   SERTHRGLYALAGLYARGTYRGLNLEULEU
8   SERARGTRPTRPASPALATYRARGLYSGLN
9   LEUGLYLEULYSASPPHESERPROLYSSER
10   GLNASPALAVALALALEUGLNGLNILELYS
11   GLUARGGLYALALEUPROMETILEASPARG
12   GLYASPILEARGGLNALAILEASPARGCYS
13   SERASNILETRPALASERLEUPROGLYALA
14   GLYTYRGLYGLNPHEGLUHISLYSALAASP
15   SERLEUILEALALYSPHELYSGLUALAGLY
16   GLYTHRVALARGGLUILEASPVAL

Samples:

sample_1: lambda_lysozyme 0 mM; H2O 95%; D2O 5%

sample_2: lambda_lysozyme 0 mM; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 0 M; pH: 5.45; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HMQC-Jsample_1isotropicsample_conditions_1
2D 15N T1 measurementsample_1isotropicsample_conditions_1
2D 15N T1 measurementsample_1isotropicsample_conditions_1
2D 15N T2 measurementsample_1isotropicsample_conditions_1
2D 15N T2 measurementsample_1isotropicsample_conditions_1
2D {1H}-15N heteronuclear NOEsample_1isotropicsample_conditions_1
2D {1H}-15N heteronuclear NOEsample_1isotropicsample_conditions_1
2D 1H-15N IPAPsample_2anisotropicsample_conditions_1

Software:

No software information available

NMR spectrometers:

  • home-built home-built 500 MHz
  • home-built home-built 750 MHz

Related Database Links:

BMRB 16664
PDB
DBJ BAI26565
EMBL CAP75477 CAQ31245 CAR03799 CBG33651 CCK47619
GB AAA96598 AAF30387 AAF31145 AAK28884 AAQ12247
PRF 711077A
REF NP_037696 NP_037753 NP_040645 NP_112069 NP_958236
SP P03706