BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 19084

Title: The Mengovirus Leader protein

Authors: Cornilescu, Claudia; Porter, Frederick; Zao, Kate Qin; Davis, Valarie; Palmenberg, Ann; Markley, John

Citation: Bacot-Davis, Valjean; Ciomperlik, J.; Basta, H.; Cornilescu, Claudia; Palmenberg, Ann. "Solution Structures of Mengovirus Leader Protein, its Phosphorylated Derivatives, and in Complex with Nuclear Transport Protein, RanGTPase"  Proc. Nat. Acad. Sci, U.S.A. ., .-. (2014).

Assembly members:
full_length_mengoL, polymer, 71 residues, Formula weight is not available

Natural source:   Common Name: Mengo virus   Taxonomy ID: 12104   Superkingdom: Viruses   Kingdom: not available   Genus/species: Cardiovirus Encephalomyocarditis virus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
full_length_mengoL: GSTAMATTMEQEICAHSMTF EECPKCSALQYRNGFYLLKY DEEWYPEELLTDGEDDVFDP DLDMEVVFETQ

Data sets:
Data typeCount
13C chemical shifts251
15N chemical shifts65
1H chemical shifts231

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1mengovirus1

Entities:

Entity 1, mengovirus 71 residues - Formula weight is not available

1   GLYSERTHRALAMETALATHRTHRMETGLU
2   GLNGLUILECYSALAHISSERMETTHRPHE
3   GLUGLUCYSPROLYSCYSSERALALEUGLN
4   TYRARGASNGLYPHETYRLEULEULYSTYR
5   ASPGLUGLUTRPTYRPROGLUGLULEULEU
6   THRASPGLYGLUASPASPVALPHEASPPRO
7   ASPLEUASPMETGLUVALVALPHEGLUTHR
8   GLN

Samples:

sample_1: full length mengoL, [U-13C; U-15N], 0.7 – 1 mM

sample_conditions_1: ionic strength: 100 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
2D-(HB)CB(CGCDCE)HEsample_1isotropicsample_conditions_1
2D-(HB)CB(CGCD)HDsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

No software information available

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 900 MHz

Related Database Links:

BMRB 19855 19857 19858
PDB
EMBL CAE84564 CAE84565 CAE84566 CAE84567
GB AAA46547 AAB59755 ABB97066 ABI15777
SP P12296 P32540