BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 19071

Title: SOLUTION STRUCTURE OF LIPIDATED GLUCAGON ANALOG IN D-TFE   PubMed: 24327962

Authors: Ward, B.; Ma, D.

Citation: Ward, Brian; Ottaway, Nickki; Perez-Tilve, Diego; Ma, Dejian; Gelfanov, Vasily; Tschop, Matthias; Dimarchi, Richard. "Peptide lipidation stabilizes structure to enhance biological function."  Mol Metab 2, 468-479 (2013).

Assembly members:
GLUCAGON_AIB2-16_LYS13-GAMMAGLU-GAMMAGLU-C16_AMIDE, polymer, 30 residues, 4269.445 Da.
entity_GGL, non-polymer, 147.129 Da.
entity_PLM, non-polymer, 256.424 Da.

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
GLUCAGON_AIB2-16_LYS13-GAMMAGLU-GAMMAGLU-C16_AMIDE: HXQGTFTSDYSKKLDXRRAQ DFVQWLMNTX

Data sets:
Data typeCount
1H chemical shifts192

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1GLUCAGON AIB2,16 LYS13-GAMMAGLU-GAMMAGLU-C16 AMIDE1
2GGL, 12
3GGL, 22
4PLM3

Entities:

Entity 1, GLUCAGON AIB2,16 LYS13-GAMMAGLU-GAMMAGLU-C16 AMIDE 30 residues - 4269.445 Da.

1   HISAIBGLNGLYTHRPHETHRSERASPTYR
2   SERLYSLYSLEUASPAIBARGARGALAGLN
3   ASPPHEVALGLNTRPLEUMETASNTHRNH2

Entity 2, GGL, 1 - C5 H9 N O4 - 147.129 Da.

1   GGL

Entity 3, PLM - C16 H32 O2 - 256.424 Da.

1   PLM

Samples:

sample_1: GLUCAGON AIB2,16 LYS13-GAMMAGLU-GAMMAGLU-C16 AMIDE 0.7 mM; sodium phosphate 20 mM

sample_conditions_1: ionic strength: 0.02 M; pH: 4.7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

X-PLOR NIH, BRUNGER - refinement

NMR spectrometers:

  • Varian Uniform NMR System 800 MHz

Related Database Links:

BMRB 19070
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