BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 19068

Title: Solution NMR Structure CTD domain of NFU1 Iron-Sulfur Cluster Scaffold Homolog from Homo sapiens, Northeast Structural Genomics Consortium (NESG) Target HR2876C

Authors: Liu, Gaohua; Xiao, Rong; Janjua, Haleema; Hamilton, Keith; Shastry, Ritu; Kohan, Eitan; Acton, Thomas; Everett, John; Pederson, Kari; Huang, Yuangpeng; Montelione, Gaetano

Citation: Liu, Gaohua; Xiao, Rong; Janjua, Haleema; Hamilton, Keith; Shastry, Ritu; Kohan, Eitan; Acton, Thomas; Everett, John; Pederson, Kari; Huang, Yuangpeng; Montelione, Gaetano. "Solution NMR Structure CTD domain of NFU1 Iron-Sulfur Cluster Scaffold Homolog from Homo sapiens, Northeast Structural Genomics Consortium (NESG) Target HR2876C"  To be published ., .-..

Assembly members:
HR2876C, polymer, 97 residues, 10818.129 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
HR2876C: MGHHHHHHSHMGSEEDDGVV AMIKELLDTRIRPTVQEDGG DVIYKGFEDGIVQLKLQGSC TSCPSSIITLKNGIQNMLQF YIPEVEGVEQVMDDESD

Data typeCount
13C chemical shifts373
15N chemical shifts91
1H chemical shifts611
residual dipolar couplings140

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HR2876C1

Entities:

Entity 1, HR2876C 97 residues - 10818.129 Da.

1   METGLYHISHISHISHISHISHISSERHIS
2   METGLYSERGLUGLUASPASPGLYVALVAL
3   ALAMETILELYSGLULEULEUASPTHRARG
4   ILEARGPROTHRVALGLNGLUASPGLYGLY
5   ASPVALILETYRLYSGLYPHEGLUASPGLY
6   ILEVALGLNLEULYSLEUGLNGLYSERCYS
7   THRSERCYSPROSERSERILEILETHRLEU
8   LYSASNGLYILEGLNASNMETLEUGLNPHE
9   TYRILEPROGLUVALGLUGLYVALGLUGLN
10   VALMETASPASPGLUSERASP

Samples:

sample_NC: HR2876C.003, [U-100% 13C; U-100% 15N], 1.0 mM; DTT 5 mM; NaCl 100 mM; Tris-HCl pH 7.5 10 mM; NaN3 0.02%

sample_NC_2: HR2876C.003, [U-100% 13C; U-100% 15N], 1.0 mM; DTT 5 mM; NaCl 100 mM; Tris-HCl pH 7.5 10 mM; NaN3 0.02%

sample_NC_3: HR2876C.003, [U-100% 13C; U-100% 15N], 1.0 mM; DTT 5 mM; NaCl 100 mM; Tris-HCl pH 7.5 10 mM; NaN3 0.02%

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_NC_3isotropicsample_conditions_1
2D 1H-13C HSQCsample_NCisotropicsample_conditions_1
3D HNCOsample_NC_3isotropicsample_conditions_1
3D CBCA(CO)NHsample_NC_3isotropicsample_conditions_1
3D HNCACBsample_NC_3isotropicsample_conditions_1
3D 1H-13C arom NOESYsample_NC_3isotropicsample_conditions_1
3D simutaneous 13C-aromatic,13C-aliphatic,15N edited 1H-1H NOESYsample_NC_3isotropicsample_conditions_1
2D 1H-15N HSQCsample_NCisotropicsample_conditions_1
3D HCCH-TOCSYsample_NC_3isotropicsample_conditions_1

Software:

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinemen,structure solution,geometry optimization

CYANA v3.0, Guntert, Mumenthaler and Wuthrich - refinement,geometry optimization,structure solution

AutoStruct v2.1, Huang, Tejero, Powers and Montelione - data analysis,refinement

AutoAssign v2.1, Zimmerman, Moseley, Kulikowski and Montelione - data analysis,chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

XEASY, Bartels et al. - data analysis,peak picking,chemical shift assignment

TOPSPIN, Bruker Biospin - collection

VNMRJ, Varian - collection

SPARKY, Goddard - data analysis

TALOS+, Shen, Cornilescu, Delaglio and Bax - geometry optimization

REDCAT, Valafar, Prestegard - geometry optimization

PSVS, Bhattacharya, Montelione - structure validation

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Varian INOVA 600 MHz
  • Varian INOVA 600 MHz

Related Database Links:

PDB
DBJ BAG36716 BAG62381 BAI46893
EMBL CAB53015 CAD98142 CAG32778
GB AAD27742 AAI05370 AAI13693 AAI13695 AAP92372
REF NP_001002755 NP_001006305 NP_001040031 NP_056515 XP_001096379
SP Q9UMS0
TPG DAA24542