BMRB Entry 19063

Title:
TIAR RRM2 chemical shifts in the apo state
Deposition date:
2013-02-27
Original release date:
2014-02-13
Authors:
Kim, Henry; Headey, Stephen; Yoga, Yano; Scanlon, Martin; Gorospe, Miriam; Wilce, Matthew; Wilce, Jacqueline
Citation:

Citation: Kim, Henry; Headey, Stephen; Yoga, Yano; Scanlon, Martin; Gorospe, Myriam; Wilce, Matthew; Wilce, Jacqueline. "Distinct binding properties of TIAR RRMs and linker region."  RNA Biol 10, 579-589 (2013).
PubMed: 23603827

Assembly members:

Assembly members:
TIAR_RRM2, polymer, 101 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET

Data sets:
Data typeCount
13C chemical shifts252
15N chemical shifts83
1H chemical shifts83

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TIAR RRM21

Entities:

Entity 1, TIAR RRM2 101 residues - Formula weight is not available

1   GLYSERPROGLYILESERGLYGLYGLYGLY
2   GLYILEPROSERASNHISPHEHISVALPHE
3   VALGLYASPLEUSERPROGLUILETHRTHR
4   GLUASPILELYSSERALAPHEALAPROPHE
5   GLYLYSILESERASPALAARGVALVALLYS
6   ASPMETALATHRGLYLYSSERLYSGLYTYR
7   GLYPHEVALSERPHETYRASNLYSLEUASP
8   ALAGLUASNALAILEVALHISMETGLYGLY
9   GLNTRPLEUGLYGLYARGGLNILEARGTHR
10   ASNTRPALATHRARGLYSPROPROALAPRO
11   LYS

Samples:

TIAR_RRM2_apo1: entity_1, [U-13C; U-15N], mM; D2O 10%; H2O 90%

TIAR_RRM2_apo2: entity_1, [U-13C; U-15N], mM; D2O 10%; H2O 90%

sample_conditions_1: ionic strength: 150 mM; pH: 6; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCTIAR_RRM2_apo2isotropicsample_conditions_1
3D HNCACBTIAR_RRM2_apo2isotropicsample_conditions_1
3D CBCA(CO)NHTIAR_RRM2_apo2isotropicsample_conditions_1
3D HNCOTIAR_RRM2_apo2isotropicsample_conditions_1
3D HN(CA)COTIAR_RRM2_apo2isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

BMRB 19064
PDB
GB AAO49722
REF XP_004599869 XP_005088544 XP_009584998

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks