BMRB Entry 19052

Title:
NMR Structure of the Complete Internal Fusion Loop mutant L529A/I544A from Ebolavirus GP2 at pH 5.5
Deposition date:
2013-02-22
Original release date:
2014-02-24
Authors:
Gregory, Sonia; Tamm, Lukas
Citation:

Citation: Gregory, Sonia; Larsson, Per; Nelson, Elizabeth; Kasson, Peter; White, Judith; Tamm, Lukas. "Ebolavirus Entry Requires a Compact Hydrophobic Fist at the Tip of the Fusion Loop."  J. Virol. ., .-. (2014).
PubMed: 24696482

Assembly members:

Assembly members:
Ebolavirus_Fusion_Loop_L529A/I544A, polymer, 54 residues, 5863.580 Da.

Natural source:

Natural source:   Common Name: Enterobacteria   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET41a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Ebolavirus_Fusion_Loop_L529A/I544A: AQPKCNPNLHYWTTQDEGAA IGAAWIPYFGPAAEGIYAEG LMHNQDGLICGLRQ

Data sets:
Data typeCount
13C chemical shifts209
15N chemical shifts49
1H chemical shifts344

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Ebolavirus_Fusion_Loop_L529A/I544A1

Entities:

Entity 1, Ebolavirus_Fusion_Loop_L529A/I544A 54 residues - 5863.580 Da.

1   ALAGLNPROLYSCYSASNPROASNLEUHIS
2   TYRTRPTHRTHRGLNASPGLUGLYALAALA
3   ILEGLYALAALATRPILEPROTYRPHEGLY
4   PROALAALAGLUGLYILETYRALAGLUGLY
5   LEUMETHISASNGLNASPGLYLEUILECYS
6   GLYLEUARGGLN

Samples:

sample_1: Ebolavirus Fusion Loop L529A/I544A, [U-15N], 600 uM; Phosphate buffer 30 mM; NaCl 50 mM; H2O 90%; D2O 10%

sample_2: Ebolavirus Fusion Loop L529A/I544A, [U-13C; U-15N], 600 uM; Phosphate buffer 30 mM; NaCl 50 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 5.5; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_2isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_2isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D H(CCO)NHsample_2isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
3D C(CO)NHsample_2isotropicsample_conditions_1
3D CBCA(CO)NHsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_2isotropicsample_conditions_1
3D 1H-13C NOESYsample_2isotropicsample_conditions_1

Software:

CNS, Brunger A. T. et.al. - refinement

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

BMRB 19383
PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks