BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 19034

Title: NMR Structure of the third RNA Recognition Motif (RRM) of U2 small nuclear ribonucleoprotein auxiliary factor (U2AF) 2

Authors: Dutta, Samit; Serrano, Pedro; Geralt, Michael; Wuthrich, Kurt

Citation: Dutta, Samit; Serrano, Pedro; Geralt, Michael; Wuthrich, Kurt. "NMR Structure of the third RNA Recognition Motif (RRM) of U2 small nuclear ribonucleoprotein auxiliary factor (U2AF) 2"  Not known ., .-..

Assembly members:
U2AF, polymer, 105 residues, 12033.749 Da.

Natural source:   Common Name: House mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
U2AF: GGHPTEVLCLMNMVLPEELL DDEEYEEIVEDVRDECSKYG LVKSIEIPRPVDGVEVPGCG KIFVEFTSVFDCQKAMQGLT GRKFANRVVVTKYCDPDSYH RRDFW

Data sets:
Data typeCount
13C chemical shifts367
15N chemical shifts102
1H chemical shifts721

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1U2AF1

Entities:

Entity 1, U2AF 105 residues - 12033.749 Da.

1   GLYGLYHISPROTHRGLUVALLEUCYSLEU
2   METASNMETVALLEUPROGLUGLULEULEU
3   ASPASPGLUGLUTYRGLUGLUILEVALGLU
4   ASPVALARGASPGLUCYSSERLYSTYRGLY
5   LEUVALLYSSERILEGLUILEPROARGPRO
6   VALASPGLYVALGLUVALPROGLYCYSGLY
7   LYSILEPHEVALGLUPHETHRSERVALPHE
8   ASPCYSGLNLYSALAMETGLNGLYLEUTHR
9   GLYARGLYSPHEALAASNARGVALVALVAL
10   THRLYSTYRCYSASPPROASPSERTYRHIS
11   ARGARGASPPHETRP

Samples:

sample_1: U2AF, [U-98% 13C; U-98% 15N], 1.2 mM; sodium phosphate 20 mM; sodium chloride 100 mM; sodium azide 5 mM; DTT 8 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.1298 M; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
4D-HACANH-APSYsample_1isotropicsample_conditions_1
5D-CBCACONH-APSYsample_1isotropicsample_conditions_1
5D-HACACONH-APSYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1

Software:

CYANA, G ntert P. - structure solution

OPAL, Luginbuhl, Guntert, Billeter and Wuthrich - refinement

TOPSPIN v3.1, Bruker Biospin - collection, processing

CARA, Keller and Wuthrich - chemical shift assignment, data analysis

j-UNIO, Herrmann, Guntert and Wuthrich - chemical shift assignment, peak picking, structure solution

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

PDB
DBJ BAC37309 BAG70075 BAG70201 BAI45669
EMBL CAA45409 CAA45874 CAA45875
GB AAH07487 AAH08740 AAH30574 AAH43071 AAH89996
PRF 1805352A
REF NP_001012496 NP_001068804 NP_001192160 NP_001302733 NP_009210
SP P26368 P26369
TPG DAA19368