BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 19017

Title: Solution structure of an intramolecular propeller-type G-quadruplex containing a single bulge   PubMed: 23521617

Authors: Thachappilly Mukundan, Vineeth

Citation: Mukundan, Vineeth Thachappilly; Phan, Anh Tuan. "Bulges in g-quadruplexes: broadening the definition of g-quadruplex-forming sequences."  J. Am. Chem. Soc. 135, 5017-5028 (2013).

Assembly members:
DNA_1, polymer, 19 residues, 6034.914 Da.

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
DNA_1: TTGTGGTGGGTGGGTGGGT

Data sets:
Data typeCount
1H chemical shifts183

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1DNA_11

Entities:

Entity 1, DNA_1 19 residues - 6034.914 Da.

1   DTDTDGDTDGDGDTDGDGDG
2   DTDGDGDGDTDGDGDGDT

Samples:

sample_1: DNA_10.1 – 2.0 mM; H2O 90%; D2O 10%; Potassium Ion 60 mM

sample_conditions_1: ionic strength: 0.05 M; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-13C JR-HMBCsample_1isotropicsample_conditions_1

Software:

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - geometry optimization, refinement

NMR spectrometers:

  • Bruker Avance 600 MHz

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