BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 19012

Title: NMR structure of hypothetical protein BT_0846 from Bacteroides thetaiotaomicron VPI-5482 (NP_809759.1)

Authors: Dutta, Samit; Serrano, Pedro; Geralt, Michael; Wuthrich, Kurt

Citation: Dutta, Samit; Serrano, Pedro; Geralt, Michael; Wuthrich, Kurt. "NMR structure of hypothetical protein BT_0846 from Bacteroides thetaiotaomicron VPI-5482 (NP_809759.1)"  Not known ., .-..

Assembly members:
BT_0846, polymer, 99 residues, 11242.420 Da.

Natural source:   Common Name: CFB group bacteria   Taxonomy ID: 818   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacteroides not available

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
BT_0846: GEDWTELNSNNIIGYWSTGI EGTHKLLSFDEDGTGSFGIY SNATPISFQMFDYKIEEGRI YIYDVYPDEKTPYYLDCKIS GTTLKVETGSEAGTYKKQK

Data sets:
Data typeCount
13C chemical shifts329
15N chemical shifts103
1H chemical shifts669

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1hypothetical protein BT_08461

Entities:

Entity 1, hypothetical protein BT_0846 99 residues - 11242.420 Da.

1   GLYGLUASPTRPTHRGLULEUASNSERASN
2   ASNILEILEGLYTYRTRPSERTHRGLYILE
3   GLUGLYTHRHISLYSLEULEUSERPHEASP
4   GLUASPGLYTHRGLYSERPHEGLYILETYR
5   SERASNALATHRPROILESERPHEGLNMET
6   PHEASPTYRLYSILEGLUGLUGLYARGILE
7   TYRILETYRASPVALTYRPROASPGLULYS
8   THRPROTYRTYRLEUASPCYSLYSILESER
9   GLYTHRTHRLEULYSVALGLUTHRGLYSER
10   GLUALAGLYTHRTYRLYSLYSGLNLYS

Samples:

sample_1: BT_0846, [U-99% 13C; U-99% 15N], 1.2 mM; sodium phosphate 20 mM; sodium chloride 50 mM; sodium azide 5 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.798 M; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
4D-HACANH-APSYsample_1isotropicsample_conditions_1
5D-HACACONH-APSYsample_1isotropicsample_conditions_1
5D-CBCACONH-APSYsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

TOPSPIN v3.1, Bruker Biospin - collection, processing

CARA, Keller and Wuthrich - chemical shift assignment, data analysis

CYANA v3.0, Guntert, Mumenthaler and Wuthrich - structure solution

jUNIO, Herrmann, Guntert and Wuthrich - chemical shift assignment, peak picking, structure solution

OPAL, Luginbuhl, Guntert, Billeter and Wuthrich - refinement

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

PDB
EMBL CUO58639
GB AAO75953 ALJ42023
REF NP_809759 WP_011107485 WP_055268830