BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 19009

Title: 40-residue beta-amyloid fibril derived from Alzheimer's disease brain   PubMed: 24034249

Authors: Lu, Jun-Xia; Qiang, Wei; Meredith, Stephen; Yau, Wai-Ming; Schweiters, Charles; Tycko, Robert

Citation: Lu, Jun-Xia; Qiang, Wei; Yau, Wai-Ming; Schweiters, Charles; Meredith, Stephen; Tycko, Robert. "Molecular Structure of beta-Amyloid Fibrils in Alzheimer's Disease Brain Tissue"  Cell 154, 1257-1268 (2013).

Assembly members:
beta-amyloid_peptide, polymer, 40 residues, 4335.893 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
beta-amyloid_peptide: DAEFRHDSGYEVHHQKLVFF AEDVGSNKGAIIGLMVGGVV

Data sets:
Data typeCount
13C chemical shifts175
15N chemical shifts41

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1beta-amyloid_peptide_11
2beta-amyloid_peptide_21
3beta-amyloid_peptide_31
4beta-amyloid_peptide_41
5beta-amyloid_peptide_51
6beta-amyloid_peptide_61
7beta-amyloid_peptide_71
8beta-amyloid_peptide_81
9beta-amyloid_peptide_91

Entities:

Entity 1, beta-amyloid_peptide_1 40 residues - 4335.893 Da.

1   ASPALAGLUPHEARGHISASPSERGLYTYR
2   GLUVALHISHISGLNLYSLEUVALPHEPHE
3   ALAGLUASPVALGLYSERASNLYSGLYALA
4   ILEILEGLYLEUMETVALGLYGLYVALVAL

Samples:

sample_1: beta-amyloid peptide, selectively and uniformly labeled samples, 1 – 2 mg

sample_conditions_1: ionic strength: 10 mM; pH: 7.4; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 13C-13C with fpRFDRsample_1solidsample_conditions_1
2D and 3D NCACXsample_1solidsample_conditions_1
2D NCOCXsample_1solidsample_conditions_1
3D NCOCXsample_1solidsample_conditions_1
2D NCACXsample_1solidsample_conditions_1
2D 13C-13C with spin diffusionsample_1solidsample_conditions_1
2D 13C-13C with RADsample_1solidsample_conditions_1
2D 13C-13C with PARsample_1solidsample_conditions_1
2D 15N-13C TEDORsample_1solidsample_conditions_1
2D 15N-13C TEDORsample_1solidsample_conditions_1
15N- and 13C-BAREsample_1solidsample_conditions_1
13C PITHIRDS-CTsample_1solidsample_conditions_1
2D 13C-13C with fpRFDRsample_1solidsample_conditions_1

Software:

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure calculations

NMR spectrometers:

  • Varian InfinityPlus 600 MHz
  • Varian Infinity 750 MHz
  • Varian InfinityPlus 400 MHz

Related Database Links:

UNP P86906.1
BMRB 11435 15775 17159 17186 17764 17793 17794 17795 17796 18052 18127 18128 18129 18131 19309 19393 25218 25289 25429 26508 26516
PDB
DBJ BAA22264 BAA84580 BAB71958 BAD51938 BAE01907
EMBL CAA30050 CAA31830 CAA39589 CAA39590 CAA39591
GB AAA35540 AAA36829 AAA51564 AAA51722 AAA51726
PIR A60045 D60045 E60045 G60045 PQ0438
PRF 1303338A 1403400A 1405204A 1507304A 1507304B
REF NP_000475 NP_001006601 NP_001013036 NP_001070264 NP_001127014
SP P05067 P53601 P79307 P86906 Q28053
TPG DAA33655