BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 19004

Title: 1H, 13C, and 15N backbone chemical shift assignments of the low-spin CN-bound yeast cytochrome c peroxidase with the C-terminal His-tag   PubMed: 23517193

Authors: Volkov, Alexander; van Nuland, Nico

Citation: Volkov, Alexander; Wohlkonig, Alexandre; Soror, Sameh; van Nuland, Nico. "Expression, Purification, Characterization, and Solution Nuclear Magnetic Resonance Study of Highly Deuterated Yeast Cytochrome c Peroxidase with Enhanced Solubility"  Biochemistry 52, 2165-2175 (2013).

Assembly members:
cytochrome_c_peroxidase, polymer, 300 residues, Formula weight is not available
PROTOPORPHYRIN IX CONTAINING FE, non-polymer, 616.487 Da.
CYANIDE ION, non-polymer, 26.017 Da.

Natural source:   Common Name: baker   Taxonomy ID: 4932   Superkingdom: not available   Kingdom: not available   Genus/species: Eukaryota Fungi

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
cytochrome_c_peroxidase: MKTLVHVASVEKGRSYEDFQ KVYNAIALKLREDDEYDNYI GYGPVLVRLAWHTSGTWDKH DNTGGSYGGTYRFKKEFNDP SNAGLQNGFKFLEPIHKEFP WISSGDLFSLGGVTAVQEMQ GPKIPWRCGRVDTPEDTTPD NGRLPDADKDADYVRTFFQR LNMNDREVVALMGAHALGKT HLKNSGYEGPWGAANNVFTN EFYLNLLNEDWKLEKNDANN EQWDSKSGYMMLPTDYSLIQ DPKYLSIVKEYANDQDKFFK DFSKAFEKLLENGITFPKDA PSPFIFKTLEEQGLHHHHHH

Data sets:
Data typeCount
1H chemical shifts267
13C chemical shifts820
15N chemical shifts267

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1protein1
2cofactor_HEME2
3cofactor_CYN3

Entities:

Entity 1, protein 300 residues - Formula weight is not available

C-terminal His-tag

1   METLYSTHRLEUVALHISVALALASERVAL
2   GLULYSGLYARGSERTYRGLUASPPHEGLN
3   LYSVALTYRASNALAILEALALEULYSLEU
4   ARGGLUASPASPGLUTYRASPASNTYRILE
5   GLYTYRGLYPROVALLEUVALARGLEUALA
6   TRPHISTHRSERGLYTHRTRPASPLYSHIS
7   ASPASNTHRGLYGLYSERTYRGLYGLYTHR
8   TYRARGPHELYSLYSGLUPHEASNASPPRO
9   SERASNALAGLYLEUGLNASNGLYPHELYS
10   PHELEUGLUPROILEHISLYSGLUPHEPRO
11   TRPILESERSERGLYASPLEUPHESERLEU
12   GLYGLYVALTHRALAVALGLNGLUMETGLN
13   GLYPROLYSILEPROTRPARGCYSGLYARG
14   VALASPTHRPROGLUASPTHRTHRPROASP
15   ASNGLYARGLEUPROASPALAASPLYSASP
16   ALAASPTYRVALARGTHRPHEPHEGLNARG
17   LEUASNMETASNASPARGGLUVALVALALA
18   LEUMETGLYALAHISALALEUGLYLYSTHR
19   HISLEULYSASNSERGLYTYRGLUGLYPRO
20   TRPGLYALAALAASNASNVALPHETHRASN
21   GLUPHETYRLEUASNLEULEUASNGLUASP
22   TRPLYSLEUGLULYSASNASPALAASNASN
23   GLUGLNTRPASPSERLYSSERGLYTYRMET
24   METLEUPROTHRASPTYRSERLEUILEGLN
25   ASPPROLYSTYRLEUSERILEVALLYSGLU
26   TYRALAASNASPGLNASPLYSPHEPHELYS
27   ASPPHESERLYSALAPHEGLULYSLEULEU
28   GLUASNGLYILETHRPHEPROLYSASPALA
29   PROSERPROPHEILEPHELYSTHRLEUGLU
30   GLUGLNGLYLEUHISHISHISHISHISHIS

Entity 2, cofactor_HEME - C34 H32 Fe N4 O4 - 616.487 Da.

1   HEM

Entity 3, cofactor_CYN - C N - 26.017 Da.

1   CYN

Samples:

sample_1: cytochrome c peroxidase, [U-13C; U-15N; U-2H], 1.1 – 1.25 mM; sodium phosphate 20 mM; sodium chloride 100 mM; D2O 5%; H2O 95%

sample_conditions_1: ionic strength: 115 mM; pH: 6; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HN(CA)CBsample_1isotropicsample_conditions_1

Software:

VNMRJ, Varian - collection

CCPN, CCPN - chemical shift assignment, data analysis, peak picking

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Varian Uniform NMR System 800 MHz

Related Database Links:

BMRB 1839 19005 19075 19076 19884 25551
PDB
DBJ GAA24787
EMBL CAA44288 CAA82145 CAY81144
GB AAA88709 AAS56247 AHY76301 AJP40095 AJS30293
REF NP_012992
SP P00431
TPG DAA09217