BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 19002

Title: Solution structure of the Core Domain (11-85) of the Murine Norovirus VPg protein.   PubMed: 23487472

Authors: Leen, Eoin; Kwok, Rex; Birtley, James; Prater, Sean; Simpson, Pete; Matthews, Steve; Marchant, Jan; Curry, Stephen

Citation: Leen, Eoin; Kwok, K. Y Rex; Birtley, James; Simpson, Peter; Subba-Reddy, Chennareddy; Chaudhry, Yasmin; Sosnovtsev, Stanislav; Green, Kim; Prater, Sean; Tong, Michael; Young, Joanna; Chung, Liliane; Marchant, Jan; Roberts, Lisa; Kao, C. Cheng; Matthews, Stephen; Goodfellow, Ian; Curry, Stephen. "Structures of the Compact Helical Core Domains of Feline Calicivirus and Murine Norovirus VPg Proteins."  J. Virol. 87, 5318-5330 (2013).

Assembly members:
MNV_VPg, polymer, 75 residues, 8876.859 Da.

Natural source:   Common Name: Murine norovirus   Taxonomy ID: 357231   Superkingdom: Viruses   Kingdom: not available   Genus/species: Murine Norovirus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
MNV_VPg: GRPGVFRTRGLTDEEYDEFK KRRESRGGKYSIDDYLADRE REEELLERDEEEAIFGDGFG LKATRRSRKAERAKL

Data sets:
Data typeCount
13C chemical shifts330
15N chemical shifts75
1H chemical shifts507

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MNV_VPg1

Entities:

Entity 1, MNV_VPg 75 residues - 8876.859 Da.

1   GLYARGPROGLYVALPHEARGTHRARGGLY
2   LEUTHRASPGLUGLUTYRASPGLUPHELYS
3   LYSARGARGGLUSERARGGLYGLYLYSTYR
4   SERILEASPASPTYRLEUALAASPARGGLU
5   ARGGLUGLUGLULEULEUGLUARGASPGLU
6   GLUGLUALAILEPHEGLYASPGLYPHEGLY
7   LEULYSALATHRARGARGSERARGLYSALA
8   GLUARGALALYSLEU

Samples:

sample_1: MNV VPg 11-85, [U-98% 13C; U-98% 15N], 590 uM; sodium phosphate 50 mM; Sodium chloride 300 mM; DTT 1 mM; H2O 90%; D2O 10%

sample_2: MNV VPg 11-85, [U-98% 13C; U-98% 15N], 710 uM; sodium chloride 360 mM; sodium azide 0.1 w/v; DTT 1 mM; sodium phosphate pH 6.5 60 mM; D2O 100%

sample_conditions_1: ionic strength: 300 mM; pH: 6.5; pressure: 1 atm; temperature: 303 K

sample_conditions_2: ionic strength: 360 mM; pH: 6.5; pressure: 1 Pa; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D H(C)CH-TOCSYsample_2isotropicsample_conditions_2
2D 1H-13C HSQC aliphaticsample_2isotropicsample_conditions_2
3D (H)CCH-TOCSYsample_2isotropicsample_conditions_2
2D 1H-13C HSQC aromaticsample_2isotropicsample_conditions_2
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D H(CC)(CO)NHsample_1isotropicsample_conditions_1
3D (H)C(CCO)NHsample_1isotropicsample_conditions_1
3D (HB)CB(CGCD)HDsample_2isotropicsample_conditions_2
3D (HB)CB(CGCDCE)HEsample_2isotropicsample_conditions_2
3D HBHA(CBCACO)NHsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_2isotropicsample_conditions_2
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution

TALOS, Cornilescu, Delaglio and Bax - geometry optimization

ARIA, Linge, O, . - structure solution

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

NMRView, Johnson, One Moon Scientific - chemical shift assignment, data analysis

MARS, Zweckstetter et al., 2004 - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

PDB
GB AAO63098 ABB90153 ABJ98943 ABS29272 ABS29274
REF YP_720001 YP_724459