BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 18985

Title: DNA containing a cluster of 8-oxo-guanine and abasic site lesion : beta anomer (6AP, 8OG14)   PubMed: 24384094

Authors: Zalesak, Jan; Jourdan, Muriel; Constant, Jean-Francois; Lourdin, Morgane

Citation: Zaleak, Jan; Lourdin, Morgane; Krej, Lumr; Constant, Jean-Francois; Jourdan, Muriel. "Structure and dynamics of DNA duplexes containing a cluster of mutagenic 8-oxoguanine and abasic site lesions."  J. Mol. Biol. 426, 1524-1538 (2014).

Assembly members:
DNA_1, polymer, 11 residues, 2965.968 Da.
not present, polymer, 11 residues, 3471.274 Da.

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
DNA_1: CGCTCXCACGC
not present: GCXTGGGAGCG

Data sets:
Data typeCount
1H chemical shifts182
31P chemical shifts20

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DNA_11
2DNA_22

Entities:

Entity 1, DNA_1 11 residues - 2965.968 Da.

1   DCDGDCDTDCORPDCDADCDG
2   DC

Entity 2, DNA_2 11 residues - 3471.274 Da.

1   DGDC8OGDTDGDGDGDADGDC
2   DG

Samples:

sample_1: DNA_1 0.88 mM; DNA_2 0.88 mM; D2O 0.88 mM

sample_conditions_1: ionic strength: 120 mM; pH: 6; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1

Software:

AMBER, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollman - structure solution

NMR spectrometers:

  • Varian UnityPlus 500 MHz

Related Database Links: