BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 18974

Title: Modified Helix 69   PubMed: 24371282

Authors: JIANG, JUN; ADURI, RAVIPRASAD; CHOW, CHRISTINE; SANTALUCIA, JOHN

Citation: Jiang, Jun; Aduri, Raviprasad; Chow, Christine; Santalucia, John. "Structure modulation of helix 69 from Escherichia coli 23S ribosomal RNA by pseudouridylations."  Nucleic Acids Res. ., .-. (2013).

Assembly members:
HELIX_69, polymer, 19 residues, 6077.715 Da.

Natural source:   Common Name: Enterobacteria   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
HELIX_69: GGCCGXAACXAXAACGGUC

Data sets:
Data typeCount
13C chemical shifts46
1H chemical shifts155

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HELIX_691

Entities:

Entity 1, HELIX_69 19 residues - 6077.715 Da.

1   GGCCGPSUAACPSU
2   APSUAACGGUC

Samples:

sample_1: HELIX_69 1.0 mM; Potassium Phosphate 10 mM; Potassium Chloride 50 mM; EDTA 0.1 mM; D2O 100%

sample_2: HELIX_69 1.0 mM; Potassium Phosphate 10 mM; Potassium Chloride 50 mM; EDTA 0.1 mM; D2O 10%; H2O 90%

sample_conditions_1: ionic strength: 70 mM; pH: 7.3; pressure: 1 atm; temperature: 310.2 K

sample_conditions_2: ionic strength: 70 mM; pH: 7.3; pressure: 1 atm; temperature: 283.2 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-13C NATURAL ABUNDANCE HMQCsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
3D 1H-1H-1H TOCSY-NOESYsample_1isotropicsample_conditions_1
2D 1H-31P HETCORsample_1isotropicsample_conditions_1
1D 31Psample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_2

Software:

SPARKY, Goddard - chemical shift assignment, data analysis

CNS v1.2, Brunger, Adams, Clore, Gros, Nilges and Read - refinement, structure solution

TOPSPIN, Bruker Biospin - collection, processing

NMR spectrometers:

  • Bruker Avance 700 MHz
  • Varian Mercury 400 MHz

Related Database Links: