BMRB Entry 18973

Title:
DNA containing a cluster of 8-oxo-guanine and abasic site lesion : alpha anomer
Deposition date:
2013-01-25
Original release date:
2014-01-06
Authors:
Zalesak, Jan; Jourdan, Muriel; Lourdin, Morgane; Constant, Jean-Francois
Citation:

Citation: Zaleak, Jan; Lourdin, Morgane; Krej, Lumr; Constant, Jean-Francois; Jourdan, Muriel. "Structure and Dynamics of DNA Duplexes Containing a Cluster of Mutagenic 8-Oxoguanine and Abasic Site Lesions."  J. Mol. Biol. ., .-. (2013).
PubMed: 24384094

Assembly members:

Assembly members:
DNA_8OG20_AP6_alpha_anomer_A_chain, polymer, 11 residues, 2965.968 Da.
DNA_8OG20_AP6_alpha_anomer_B_chain, polymer, 11 residues, 3471.274 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
DNA_8OG20_AP6_alpha_anomer_A_chain: CGCTCXCACGC
DNA_8OG20_AP6_alpha_anomer_B_chain: GCGTGGGAXCG

Data sets:
Data typeCount
1H chemical shifts179
31P chemical shifts20

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1DNA (5'-D(*CP*GP*CP*TP*CP*CP*AP*CP*GP*C)-3')1
2DNA (5'-D(*GP*CP*GP*TP*GP*GP*GP*AP*(8OG)P*CP*G)-3')2

Entities:

Entity 1, DNA (5'-D(*CP*GP*CP*TP*CP*CP*AP*CP*GP*C)-3') 11 residues - 2965.968 Da.

1   DCDGDCDTDCORPDCDADCDG
2   DC

Entity 2, DNA (5'-D(*GP*CP*GP*TP*GP*GP*GP*AP*(8OG)P*CP*G)-3') 11 residues - 3471.274 Da.

1   DGDCDGDTDGDGDGDA8OGDC
2   DG

Samples:

sample_1: DNA (5'-D(*CP*GP*CP*TP*CP*CP*AP*CP*GP*C)-3') 0.55 mM; DNA (5'-D(*GP*CP*GP*TP*GP*GP*GP*AP*(8OG)P*CP*G)-3') 0.55 mM

sample_conditions_1: ionic strength: 120 mM; pH: 6; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1

Software:

AMBER, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollman - structure solution

NMR spectrometers:

  • Varian UnityPlus 500 MHz