BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 18964

Title: Characterization of a Novel Alpha4/6-Conotoxin TxIC from Conus textile that Potently Blocks alpha3beta4 Nicotinic Acetylcholine Receptors   PubMed: 24200193

Authors: Luo, Sulan; Zhangsun, Dongting; Zhu, Xiaopeng; Wu, Yong; Hu, Yuanyan; Christensen, Sean; Akcan, Muharrem; Craik, David; McIntosh, J. Michael

Citation: Luo, Sulan; Zhangsun, Dongting; Zhu, Xiaopeng; Wu, Yong; Hu, Yuanyan; Christensen, Sean; Harvey, Peta; Akcan, Muharrem; Craik, David; McIntosh, J. Michael. "Characterization of a Novel -Conotoxin TxID from Conus textile That Potently Blocks Rat 34 Nicotinic Acetylcholine Receptors."  J. Med. Chem. 56, 9655-9663 (2013).

Assembly members:
TxIC, polymer, 16 residues, 1493.806 Da.

Natural source:   Common Name: Cloth-of-gold Cone   Taxonomy ID: 6494   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Conus Textile

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
TxIC: GCCSHPVCSAMSPICX

Data sets:
Data typeCount
1H chemical shifts77

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Novel Alpha4/6-Conotoxin TxIC1

Entities:

Entity 1, Novel Alpha4/6-Conotoxin TxIC 16 residues - 1493.806 Da.

1   GLYCYSCYSSERHISPROVALCYSSERALA
2   METSERPROILECYSNH2

Samples:

sample_1: Conotoxin ~3 mg; H2O 70%; Trifluoroethanol 30%

sample_conditions_1: ionic strength: 0 M; pH: 3.5; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

CNSSOLVE, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

MolProbity, Richardson - Structure validation

PROMOTIF, Hutchinson, E. G., and Thornton, J. M - Structure validation

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

PDB
EMBL CCN27219
GB AFD18484