BMRB Entry 18920

Title:
Solution structure of Entamoeba histolytica HP1 chromodomain
Deposition date:
2012-12-28
Original release date:
2014-05-12
Authors:
Wiggins, Kimberly; Ren, Xiaobai; Khorasanizadeh, Sepideh
Citation:

Citation: Ali, Ibne; Wiggins, Kimberly; Ren, Xiaobai; Sherman, Nick; Petri, William; Khorasanizadeh, Sepideh. "Characterization of putative readers of epigenetic methyl-marks from Entamoeba histolytica"  .

Assembly members:

Assembly members:
entity, polymer, 67 residues, 8223.235 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET46

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts234
15N chemical shifts65
1H chemical shifts340

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Entamoeba histolytica HP1 chromodomain1

Entities:

Entity 1, Entamoeba histolytica HP1 chromodomain 67 residues - 8223.235 Da.

1   METHISHISHISHISHISHISGLUVALPRO
2   GLNTHRPHEGLUVALGLUARGILEVALARG
3   LYSLYSILEVALHISGLYASNTHRSERTYR
4   LEUVALLYSTRPLYSASNTYRSERSERLYS
5   ASPASNTHRTRPGLUTHRGLUASPASPILE
6   ARGTHRLYSTYRGLYASPLEUVALASPASP
7   PHEGLULYSASNGLNLYSLYS

Samples:

sample_1: entity, [U-15N], mM; sodium phosphate 50 mM; sodium chloride 25 mM; DTT 1 mM; D2O, [U-100% 2H], 5%

sample_2: entity, [U-13C; U-15N], mM; sodium phosphate 50 mM; sodium chloride 25 mM; DTT 1 mM; D2O, [U-100% 2H], 5%

sample_conditions_1: ionic strength: 75 mM; pH: 6.0; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1
3D CBCA(CO)NHsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_2isotropicsample_conditions_1
3D 1H-13C NOESYsample_2isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment, data analysis, peak picking

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

TOPSPIN, Bruker Biospin - collection

CYANA, Guntert, Mumenthaler and Wuthrich - data analysis, refinement, structure solution

TALOS, Cornilescu, Delaglio and Bax - data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

PDB
GB EAL47287 EMD45296 EMH75139 EMS14461 ENY59971
REF XP_652673

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks