BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 18907

Title: Solution structure of Duplex DNA

Authors: Ghosh, A.; Kar, R.; Jana, J.; Biswas, A.; Ghosh, S.; Kumar, D.; Chatterjee, S.; Bhunia, A.

Citation: Ghosh, A.; Kar, R.; Jana, J.; Biswas, A.; Ghosh, S.; Kumar, D.; Chatterjee, S.; Bhunia, A.. "Structural Insights of DNA Duplex Stabilization by Potent Antimicrobial Peptide Indolicidin."  Not known ., .-..

Assembly members:
DNA_GCGCATGCTACGCG, polymer, 14 residues, 3952.606 Da.
DNA_CGCGTAGCATGCGC, polymer, 14 residues, 3952.606 Da.

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: Chemically synthetic DNA   Host organism: Chemically synthetic DNA

Entity Sequences (FASTA):
DNA_GCGCATGCTACGCG: GCGCATGCTACGCG
DNA_CGCGTAGCATGCGC: CGCGTAGCATGCGC

Data sets:
Data typeCount
1H chemical shifts225

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1GCGCATGCTACGCG1
2CGCGTAGCATGCGC2

Entities:

Entity 1, GCGCATGCTACGCG 14 residues - 3952.606 Da.

1   DGDCDGDCDADTDGDCDTDA
2   DCDGDCDG

Entity 2, CGCGTAGCATGCGC 14 residues - 3952.606 Da.

1   DCDGDCDGDTDADGDCDADT
2   DGDCDGDC

Samples:

sample_1: GCGCATGCTACGCG 1 mM; CGCGTAGCATGCGC 1 mM

sample_conditions_1: pH: 7.2; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H COSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

AMBER v2.1, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollman - refinement

NMR spectrometers:

  • Bruker Avance 500 MHz
  • Bruker DRX 800 MHz

Related Database Links: