BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 18905

Title: NMR structure of the RRM2 domain of the protein RBM10 from homo sapiens

Authors: Serrano, Pedro; Geralt, Michael; Dutta, Samit; Wuthrich, Kurt; Wrobel, Russell; Makino, Shi-Ichi; Misenhiemer, Tina; Markley, John; Fox, Brian

Citation: Serrano, Pedro; Wuthrich, Kurt. "NMR structure of the RRM2 domain of the protein RBM10 from homo sapiens"  Not known ., .-..

Assembly members:
entity, polymer, 131 residues, 13784.618 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: obtained from a collaborator   Host organism: wheat germ - cell free

Entity Sequences (FASTA):
entity: QAQGVLASQALSQGSEPSSE NANDTIILRNLNPHSTMDSI LGALAPYAVLSSSNVRVIKD KQTQLNRGFAFIQLSTIEAA QLLQILQALHPPLTIDGKTI NVEFAKGSKRDMASNEGSRI SAASVASTAIA

Data sets:
Data typeCount
13C chemical shifts446
15N chemical shifts124
1H chemical shifts766

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RRM2 domain of the protein RBM10 from homo sapiens1

Entities:

Entity 1, RRM2 domain of the protein RBM10 from homo sapiens 131 residues - 13784.618 Da.

1   GLNALAGLNGLYVALLEUALASERGLNALA
2   LEUSERGLNGLYSERGLUPROSERSERGLU
3   ASNALAASNASPTHRILEILELEUARGASN
4   LEUASNPROHISSERTHRMETASPSERILE
5   LEUGLYALALEUALAPROTYRALAVALLEU
6   SERSERSERASNVALARGVALILELYSASP
7   LYSGLNTHRGLNLEUASNARGGLYPHEALA
8   PHEILEGLNLEUSERTHRILEGLUALAALA
9   GLNLEULEUGLNILELEUGLNALALEUHIS
10   PROPROLEUTHRILEASPGLYLYSTHRILE
11   ASNVALGLUPHEALALYSGLYSERLYSARG
12   ASPMETALASERASNGLUGLYSERARGILE
13   SERALAALASERVALALASERTHRALAILE
14   ALA

Samples:

sample_1: entity, [U-98% 13C; U-98% 15N], 0.6 mM; sodium chloride 100 mM; sodium acetate, [U-99% 2H], 10 mM; sodium azide 5 mM; DTT 2 mM; H2O 95%; D20 5%

sample_conditions_1: ionic strength: 0.220 M; pH: 5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
4D APSY HACANHsample_1isotropicsample_conditions_1
5D APSY CBCACONHsample_1isotropicsample_conditions_1
5D APSY HACACONHsample_1isotropicsample_conditions_1

Software:

CYANA, G??ntert P. - refinement

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

PDB
DBJ BAA12144 BAC31087 BAC40753 BAE39715 BAF85447
GB AAH03089 AAH93519 AIC55382 EAW59284 EAW59287
REF NP_001161248 NP_001191395 NP_690595 NP_690600 XP_003135120
SP P70501
TPG DAA12903