BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 18903

Title: Relaxation study of Delta subunit of RNA polymerase from Bacillus subtilis   PubMed: 24515886

Authors: Kaderavek, Pavel; Sklenar, Vladimir; Zidek, Lukas; Rabatinova, Alzbeta; Krasny, Libor

Citation: Kadeavek, Pavel; Zapletal, Vojtch; Rabatinova, Albeta; Krasny, Libor; Sklena, Vladimir; Zidek, Luka. "Spectral density mapping protocols for analysis of molecular motions in disordered proteins."  J. Biomol. NMR ., .-. (2014).

Assembly members:
delta, polymer, 172 residues, Formula weight is not available

Natural source:   Common Name: Bacillus subtilis   Taxonomy ID: 1423   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacillus subtilis

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
delta: GIKQYSQEELKEMALVEIAH ELFEEHKKPVPFQELLNEIA SLLGVKKEELGDRIAQFYTD LNIDGRFLALSDQTWGLRSW YPYDQLDEETQPTVKAKKKK AKKAVEEDLDLDEFEEIDED DLDLDEVEEELDLEADDFDE EDLDEDDDDLEIEEDIIDED DEDYDDEEEEIK

Data sets:
Data typeCount
dipole-CSA cross correlation relaxation values264
heteronuclear NOE values96
T1 relaxation values98
T2 relaxation values203

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1delta1

Entities:

Entity 1, delta 172 residues - Formula weight is not available

1   GLYILELYSGLNTYRSERGLNGLUGLULEU
2   LYSGLUMETALALEUVALGLUILEALAHIS
3   GLULEUPHEGLUGLUHISLYSLYSPROVAL
4   PROPHEGLNGLULEULEUASNGLUILEALA
5   SERLEULEUGLYVALLYSLYSGLUGLULEU
6   GLYASPARGILEALAGLNPHETYRTHRASP
7   LEUASNILEASPGLYARGPHELEUALALEU
8   SERASPGLNTHRTRPGLYLEUARGSERTRP
9   TYRPROTYRASPGLNLEUASPGLUGLUTHR
10   GLNPROTHRVALLYSALALYSLYSLYSLYS
11   ALALYSLYSALAVALGLUGLUASPLEUASP
12   LEUASPGLUPHEGLUGLUILEASPGLUASP
13   ASPLEUASPLEUASPGLUVALGLUGLUGLU
14   LEUASPLEUGLUALAASPASPPHEASPGLU
15   GLUASPLEUASPGLUASPASPASPASPLEU
16   GLUILEGLUGLUASPILEILEASPGLUASP
17   ASPGLUASPTYRASPASPGLUGLUGLUGLU
18   ILELYS

Samples:

sample_1: delta, [U-100% 15N], 8.8 mg/mL; D2O, [U-2H], 10%; sodium chloride 10 mM; sodium azide 50 uM; phosphate buffer 20 mM; H2O 90%

sample_conditions_1: ionic strength: 30 mM; pH: 6.6; pressure: 1 atm; temperature: 300.15 K

Experiments:

NameSampleSample stateSample conditions
2D R1 15N,1H correlationsample_1isotropicsample_conditions_1
2D R2 15N,1H correlationsample_1isotropicsample_conditions_1
2D NOE 15N,1H correlationsample_1isotropicsample_conditions_1
2D 15N (coupled),1H correlationsample_1isotropicsample_conditions_1
2D 15N,1H correlation - transverse cross-correlated cross-relaxation (symmetrical reconversion)sample_1isotropicsample_conditions_1
2D 15N,1H correlation - longitudinal cross-correlated cross-relaxation (symmetrical reconversion)sample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - data analysis

Relax, d, Gooley,, 18903 - data analysis

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

NMR spectrometers:

  • Bruker Avance 500 MHz
  • Bruker Avance 600 MHz

Related Database Links:

BMRB 16635 16912 19284
PDB
DBJ BAM55799 BAM59811
EMBL CAA89869 CAB15744 CCU60814 CEI59524 CEJ79381
GB AAA22710 ADM39706 ADV94526 AEP92774 AFI30331
REF NP_391597 WP_003221959 WP_003227609 WP_003235832 WP_003243661
SP P12464