BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 18884

Title: CD79b cytosolic domain   PubMed: 23667548

Authors: Mayzel, Maxim

Citation: Isaksson, Linnea; Mayzel, Maxim; Saline, Maria; Pedersen, Anders; Brutscher, Bernhard; Karlsson, Goran; Orekhov, Vladislav. "Highly efficient NMR assignment of intrinsically disordered proteins: application to B- and T cell receptor domains"  PLoS One 8, e62947-e62947 (2013).

Assembly members:
CD79b, polymer, 51 residues, 5702.0248 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: cell free synthesis   Host organism: Wheat germ - cell freeE. coli - cell free

Entity Sequences (FASTA):
CD79b: SLLDKDDSKAGMEEDHTYEG LDIDQTATYEDIVTLRTGEV KWSVGEHPGQE

Data sets:
Data typeCount
13C chemical shifts145
15N chemical shifts48
1H chemical shifts48

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CD79b1

Entities:

Entity 1, CD79b 51 residues - 5702.0248 Da.

1   SERLEULEUASPLYSASPASPSERLYSALA
2   GLYMETGLUGLUASPHISTHRTYRGLUGLY
3   LEUASPILEASPGLNTHRALATHRTYRGLU
4   ASPILEVALTHRLEUARGTHRGLYGLUVAL
5   LYSTRPSERVALGLYGLUHISPROGLYGLN
6   GLU

Samples:

Native: CD79b, [U-100% 13C; U-100% 15N], 0.1 mM; DTT 2.0 mM; EDTA 1.0 mM; potassiup phosphate 20.0 mM; azide 0.02%; Complete EDTA-free 1 x; H2O 90%; D2O 10%

1: ionic strength: 0.020 M; pH: 6.800; pressure: 1.000 atm; temperature: 298.000 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQC/HMQCNativeisotropic1
3D HN(CA)CONativeisotropic1
3D HNCANativeisotropic1
3D HNCACBNativeisotropic1
3D HNCONativeisotropic1
3D HN(CO)CANativeisotropic1
3D HN(CO)CACBNativeisotropic1

Software:

AutoAssign v2.3.0, Zimmerman, Moseley, Kulikowski and Montelione - Automatic assignment

ANALYSIS v2.2, CCPN - data analysis

MDDNMR v2.1, Swedish NMR Center, University of Gothenburg - Spectrum processing

TANSY v1.0, Swedish NMR Center, University of Gothenburg - Spectra processing & analysis

NMRPipe vany, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - Spectrum processing

NMR spectrometers:

  • Varian UnityInova 600 MHz

Related Database Links:

UNP P40259
BMRB 19650 19651
DBJ BAA25652 BAD96674 BAD96930 BAG36353
EMBL CAA58522
GB AAA58387 AAA64459 AAA72424 AAB24822 AAC60654
REF NP_000617 NP_001035022 NP_001230841 NP_001248454 NP_067613
SP P40259
TPG DAA18410