BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 18872

Title: ID3 stem

Authors: Popovic, Milena; Greenbaum, Nancy

Citation: Popovic, Milena; Greenbaum, Nancy. "ID3 stem"  Not known ., .-..

Assembly members:
RNA_GGGUGUA, polymer, 7 residues, Formula weight is not available
RNA_AGCACCC, polymer, 7 residues, Formula weight is not available

Natural source:   Common Name: baker   Taxonomy ID: 4932   Superkingdom: not available   Kingdom: not available   Genus/species: Eukaryota Fungi

Experimental source:   Production method: enzymatic semisynthesis   Host organism: Saccharomyces cerevisiae

Entity Sequences (FASTA):
RNA_GGGUGUA: GGGUGUA
RNA_AGCACCC: AGCACCC

Data sets:
Data typeCount
13C chemical shifts59
1H chemical shifts73

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1GGGUGUA1
2AGCACCC2

Entities:

Entity 1, GGGUGUA 7 residues - Formula weight is not available

1   GGGUGUA

Entity 2, AGCACCC 7 residues - Formula weight is not available

1   AGCACCC

Samples:

sample_1: RNA_GGGUGUA 1 mM; RNA_AGCACCC 1 mM; sodium chloride 80 mM; EDTA 0.1 mM

sample_conditions_1: ionic strength: 0.08 M; pH: 6.4; pressure: 1 atm; temperature: 288 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

CCPN, CCPN - chemical shift assignment

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links: